原创力文档role of rho kinase in microvascular damage following cerebral ischemia reperfusion in ratsρ激酶的作用在脑缺血再灌注后大鼠微血管损害.pdfVIP
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role of rho kinase in microvascular damage following cerebral ischemia reperfusion in ratsρ激酶的作用在脑缺血再灌注后大鼠微血管损害
Int. J. Mol. Sci. 2011, 12, 1222-1231; doi:10.3390/ijm
OPEN ACCESS
International Journal of
Molecular Sciences
ISSN 1422-0067
/journal/ijms
Article
Role of Rho Kinase in Microvascular Damage Following
Cerebral Ischemia Reperfusion in Rats
Kang Liu 1,2,†, Zhen Li 1,†, Tao Wu 1 and Suju Ding 1,*
1 Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai
200433, China; E-Mails: lllkkkjjj12345@ (K.L.); wutao.doctor@ (T.W.);
jxleezhen@163.com (Z.L.)
2 Department of Neurology, the 456th Hospital of PLA, Jinan 250031, China
† These two authors contributed equally to this work.
* Author to whom correspondence should be addressed; E-Mail: dingsuju@;
Tel.: +86-21 Fax: +86-21
Received: 12 December 2010; in revised form: 21 January 2011 / Accepted: 7 February 2011/
Published: 18 February 2011
Abstract: Rho kinase (ROCK) is a well-known downstream effector of Rho and plays an
important role in various physiopathological processes. In this study, we aim to investigate
the correlation between ROCK and microvascular damage in rat brain subjected to middle
cerebral artery occlusion (MCAO) and reperfusion, and to elucidate the mechanisms
underlying the microvascular damage. ROCK and matrix metalloproteinase 9 (MMP9)
mRNA levels were determined by real time quantitative PCR, Laminin was detected by
immunofluorescence and Blood Brain Barrier
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