role of surface chemistry in protein remodeling at the cell-material interface表面化学的作用在蛋白质重构测量界面.pdfVIP

Role of Surface Chemistry in Protein Remodeling at the Cell-Material Interface ´ 1. 1,2. ´ ´ 1 1 Virginia Llopis-Hernandez , Patricia Rico , Jose Ballester-Beltran , David Moratal , Manuel ´ ´ 1,2,3* Salmeron-Sanchez ´ ´ 1 Center for Biomaterials and Tissue Engineering, Universidad Politecnica de Valencia, Valencia, Spain, 2 CIBER de Bioingenierıa, Biomateriales y Nanomedicina (CIBER- ´ ´ BBN), Valencia, Spain, 3 Regenerative Medicine Unit, Centro de Investigacion Prıncipe Felipe, Valencia, Spain Abstract Background: The cell-material interaction is a complex bi-directional and dynamic process that mimics to a certain extent the natural interactions of cells with the extracellular matrix. Cells tend to adhere and rearrange adsorbed extracellular matrix (ECM) proteins on the material surface in a fibril-like pattern. Afterwards, the ECM undergoes proteolytic degradation, which is a mechanism for the removal of the excess ECM usually approximated with remodeling. ECM remodeling is a dynamic process that consists of two opposite events: assembly and degradation. Methodology/Principal Findings: This work investigates matrix protein dynamics on mixed self-assembled monolayers (SAMs) of –OH and –CH3 terminated alkanethiols. SAMs assembled on gold are highly ordered organic surfaces able to provide different chemical functionalitie