role of the mannose receptor (cd206) in innate immunity to ricin toxin甘露糖受体的作用(cd206)先天免疫蓖麻毒蛋白毒素.pdfVIP

Toxins 2011, 3, 1131-1145; doi:10.3390/toxins3091131 OPEN ACCESS toxins ISSN 2072-6651 /journal/toxins Article Role of the Mannose Receptor (CD206) in Innate Immunity to Ricin Toxin 1 1 1,2 1 Emily Gage , Maria O. Hernandez , Joanne M. O’Hara , Elizabeth A. McCarthy and Nicholas J. Mantis 1,2,* 1 Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA; E-Mails: gage.emily@ (E.G.); moh01@ (M.O.H.); joanne.m.ohara@gmail .com (J.M.O.); emccart2@ (E.A.M.) 2 Department of Biomedical Sciences, University at Albany School of Public Health, Albany, NY 12201, USA * Author to whom correspondence should be addressed; E-Mail: nmantis@; Tel.: +1-518-473-7487; Fax: +1-518-402-4773. Received: 27 July 2011; in revised form: 26 August 2011 / Accepted: 30 August 2011 / Published: 9 September 2011 Abstract: The entry of ricin toxin into macrophages and certain other cell types in the spleen and liver results in toxin-induced inflammation, tissue damage and organ failure. It has been proposed that uptake of ricin into macrophages is facilitated by the mannose receptor (MR; CD206), a C-type lectin known to recognize the oligosaccharide side chains on ricin’s A (RTA) and B (RTB) subunits. In this study, we confirmed that the MR does indeed promote ricin binding, uptake and killing of monocytes in vitro. To assess the role