routine use of microbial whole genome sequencing in diagnostic and public health microbiology常规使用的微生物全基因组测序诊断和公共卫生微生物学.pdfVIP

Opinion Routine Use of Microbial Whole Genome Sequencing in Diagnostic and Public Health Microbiology ¨ 1,2 2 1,2 3 Claudio U. Koser *, Matthew J. Ellington , Edward J. P. Cartwright , Stephen H. Gillespie , 2 2 4 4 4 Nicholas M. Brown , Mark Farrington , Matthew T. G. Holden , Gordon Dougan , Stephen D. Bentley , Julian Parkhill4, Sharon J. Peacock1,2,4,5 1 Department of Medicine, University of Cambridge, Addenbrooke’s Hospital, Cambridge, United Kingdom, 2 Clinical Microbiology and Public Health Laboratory, Health Protection Agency Microbiology Services, Addenbrooke’s Hospital, Cambridge, United Kingdom, 3 The Medical School University of St. Andrews, North Haugh, St. Andrews Fife, United Kingdom, 4 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom, 5 Department of Pathology, University of Cambridge, Cambridge, United Kingdom Whole genome sequencing (WGS) prom- WGS as a routine tool for diagnostic and main stages, starting with detection (or ises to be transformative for the practice of public health microbiology, together with not) of a pathogen in a sample. If a clinical microbiology, and the rapidly falling the technical and infrastructure require- clinically relevant pathogen is detected, cost and turnaround time mean that this ments for this to be realised. The health- then this may be further tested for will become a viable te