ultrafast mid-ir laser scalpel protein signals of the fundamental limits to minimally invasive surgery不仅超速中激光手术刀蛋白质信号微创手术的基本限制.pdfVIP

Ultrafast Mid-IR Laser Scalpel: Protein Signals of the Fundamental Limits to Minimally Invasive Surgery 1 2 2 1 1 Saeid Amini-Nik , Darren Kraemer , Michael L. Cowan , Keith Gunaratne , Puviindran Nadesan , 1 2 Benjamin A. Alman , R. J. Dwayne Miller * 1 Program in Developmental and Stem Cell Biology, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada, 2 Departments of Chemistry and Physics and the Institute for Optical Sciences, University of Toronto, Toronto, Ontario, Canada Abstract Lasers have in principle the capability to cut at the level of a single cell, the fundamental limit to minimally invasive procedures and restructuring biological tissues. To date, this limit has not been achieved due to collateral damage on the macroscale that arises from thermal and shock wave induced collateral damage of surrounding tissue. Here, we report on a novel concept using a specifically designed Picosecond IR Laser (PIRL) that selectively energizes water molecules in the tissue to drive ablation or cutting process faster than thermal exchange of energy and shock wave propagation, without plasma formation or ionizing radiation effects. The targeted laser process imparts the least amount of energy in the remaining tissue without any of the deleterious photochemical or photothermal effects that accompanies other laser wavelengths and pulse parameters. Full thickness incisional and excisional wounds were generated in CD1 mice using the Picosecond IR Laser, a conventional surgical laser (DELight Er:YAG) or mechanical surgical tools. Transmission and scanning electron microscopy showed that the PIRL laser pro