原创力文档use of rbcl and trnl-f as a two-locus dna barcode for identification of nw-european ferns an ecological perspective使用rbcl和trnl-f two-locus dna条形码作为识别nw-european蕨类植物生态的视角.pdfVIP
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use of rbcl and trnl-f as a two-locus dna barcode for identification of nw-european ferns an ecological perspective使用rbcl和trnl-f two-locus dna条形码作为识别nw-european蕨类植物生态的视角
Use of rbcL and trnL-F as a Two-Locus DNA Barcode for
Identification of NW-European Ferns: An Ecological
Perspective
1 1 1 2 2
G. Arjen de Groot *, Heinjo J. During , Jan W. Maas , Harald Schneider , Johannes C. Vogel , Roy H. J.
Erkens1
1 Ecology and Biodiversity Group, Institute of Environmental Biology, Utrecht University, Utrecht, The Netherlands, 2 Department of Botany, Natural History Museum,
London, United Kingdom
Abstract
Although consensus has now been reached on a general two-locus DNA barcode for land plants, the selected combination
of markers (rbcL + matK) is not applicable for ferns at the moment. Yet especially for ferns, DNA barcoding is potentially of
great value since fern gametophytes—while playing an essential role in fern colonization and reproduction—generally lack
the morphological complexity for morphology-based identification and have therefore been underappreciated in ecological
studies. We evaluated the potential of a combination of rbcL with a noncoding plastid marker, trnL-F, to obtain DNA-
identifications for fern species. A regional approach was adopted, by creating a reference database of trusted rbcL and trnL-F
sequences for the wild-occurring homosporous ferns of NW-Europe. A combination of parsimony analyses and distance-
based analyses was performed to evaluate the discriminatory power of the two-region barcode. DNA was successfully
extracted from 86 tiny fern gametophytes and was used as a test case for the performance of DNA-based identification.
Primer universality proved high for both markers. Based on the combined rbcL + trnL-F dataset, all genera as well as all
species with non-equal chloroplast genomes formed their own well supported monophyletic clade, indic
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