原创力文档use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens使用敏感,广谱分子检测和人工气道上皮文化呼吸道病原体的检测.pdfVIP
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use of sensitive, broad-spectrum molecular assays and human airway epithelium cultures for detection of respiratory pathogens使用敏感,广谱分子检测和人工气道上皮文化呼吸道病原体的检测
Use of Sensitive, Broad-Spectrum Molecular Assays and
Human Airway Epithelium Cultures for Detection of
Respiratory Pathogens
1 . 1. 2,4 1 3
Krzysztof Pyrc * , Karol Stoz_ek , Krzysztof Wojcik , Katarzyna Gawron , Slawomir Zeglen , Wojciech
3 3 3 4 4
Karolak , Jacek Wojarski , Marek Ochman , Magdalena Hubalewska-Mazgaj , Grazyna Bochenek ,
4 3 4 1,5
Marek Sanak , Marian Zembala , Andrzej Szczeklik , Jan Potempa
1 Microbiology Department, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland, 2 Division of Cell Biophysics, Faculty of
Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland, 3 Department of Cardiac Surgery and Transplantology, Silesian Center for Heart
Diseases, Zabrze, Poland, 4 Department of Medicine, Jagiellonian University Medical College, Krakow, Poland, 5 Oral Health and Systemic Disease Research Group, School
of Dentistry, University of Louisville, Louisville, Kentucky, United States of America
Abstract
Rapid and accurate detection and identification of viruses causing respiratory tract infections is important for patient care
and disease control. Despite the fact that several assays are available, identification of an etiological agent is not possible in
,30% of patients suffering from respiratory tract diseases. Therefore, the aim of the current study was to develop a
diagnostic set for the detection of respiratory viruses with sensitivity as low as 1–10 copies per
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