use of postmortem human dura mater and scalp for deriving human fibroblast cultures使用后期人工硬脑膜和头皮派生人类成纤维细胞的文化.pdfVIP

Use of Postmortem Human Dura Mater and Scalp for Deriving Human Fibroblast Cultures 1 1 1 1 2 Lindsay A. Bliss , Malik R. Sams , Amy Deep-Soboslay , Renee Ren-Patterson , Andrew E. Jaffe , 2 2 1 2 Josh G. Chenoweth , Amritha Jaishankar , Joel E. Kleinman , Thomas M. Hyde * 1 Section on Neuropathology, Clinical Brain Disorders Branch, Division of Intramural Research Programs, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, United States of America, 2 Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland, United States of America Abstract Fibroblasts can be collected from deceased individuals, grown in culture, reprogrammed into induced pluripotent stem cells (iPSCs), and then differentiated into a multitude of cell types, including neurons. Past studies have generated iPSCs from somatic cell biopsies from either animal or human subjects. Previously, fibroblasts have only been successfully cultured from postmortem human skin in two studies. Here we present data on fibroblast cell cultures generated from 146 scalp and/or 53 dura mater samples from 146 postmortem human brain donors. In our overall sample, the odds of successful dural culture was almost two-fold compared with scalp (OR = 1.95, 95% CI: [1.01, 3.9], p = 0.047). Using a paired design within subjects for whom both tissues were available for culture (n = 53), the odds of success for culture in dura was 16-fold as compared to scalp (OR = 16.0, 95% CI: [2.1–120.6], p = 0.0007). Unattended death, tissue donation source, longer postmortem interval (PMI), and higher body mass index (BMI) were ass