软肝颗粒对四氯化碳复合法致肝纤维化大鼠血清丙氨酸氨基转移酶等.docVIP

软肝颗粒对四氯化碳复合法致肝纤维化大鼠血清丙氨酸氨基转移酶等 　 作者：毕红征 薛敬礼 黄国钧 章金涛 王纯耀 【摘要】 目的研究软肝颗粒（HG颗粒）对CCl4（四氯化碳）复合法致肝纤维化大鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶(AST)、总蛋白(TP )、白蛋白（ALb）及球蛋白（Glob）含量的影响。方法70只SD大鼠，除空白对照组10只外，其余各组大鼠皮下注射CCl4，同时给予高脂饲料和20%乙醇喂养6周建立肝纤维化大鼠模型，存活大鼠用分组软件按性别、体重随机分为5组，分别予HG颗6，3，1.5 g/kg 3个剂量组、复方鳖甲软肝片1.0 g/kg灌胃治疗4周。对照观察两药物对模型大鼠血清ALT，AST，TP，Alb及Glob的影响。结果模型大鼠血清ALT、AST活力显著增高，血清TP含量略升高，Alb含量显著降低，Glob含量显著升高。HG颗粒治疗后降低ALT和AST活性，降低TP和Glob含量，增加Alb含量，复方鳖甲软肝片亦可降低ALT，AST活性，降低TP和Glob含量，增加Alb含量。综合疗效HG颗粒优于复方鳖甲软肝片。结论HG颗粒能有效治疗CCl4复合法所致肝纤维化大鼠。 【关键词】 HG颗粒； 丙氨酸氨革转移酶； 天冬氨酸氨基转移酶； 总蛋白； 白蛋白； 球蛋白 　　Abstract：ObjectiveTo explore the effects of HGKL on ALT，AST，TP，Alb and Glob in blood serum of HF model rats. MethodsSeventy SD rats excluding ten in blank group, were injected hypodermicly by carbon tetrar-chloride,at the same time they were fed with the high fat food and 20% ethyl alcohol for six weeks to establish HF model rats.Existing rats were divided into five groups based on sex and weight by grouping software.The HF rats were administered HGKL 6，3，1.5 g/kg and FFBJRGP 1.0g/kg respectivly for four weeks. ALT，AST，TP，Alb and Glob in blood serum were determined.ResultsActivities of ALT and AST in blood serum of model rats increased markedly as compared with blank group. The content of TP increased softly,Alb decreased markedly,Glob increased markedly. Compared with model group, activities of ALT and AST，and the contents of TP and Glob in HGKL groups decreased，Alb increased. Activities of ALT and AST in FFBJRGP group decreased，contents of TP and Glob decreased，contents of Alb increased.The comprehensive action of HGKL was better than that of FFBJRGP. ConclusionHGKL has protective effects on HF rats induced by CCl4 compounded other factors. 　　Key words：HGKL； ALT； AST； TP； Alb； Glob 　　肝纤维化(hepatic fibrosis)是各种慢性肝病进展中由于肝内纤维生成与降解失衡，导致过多的胶原在肝内沉积所致，常伴有炎症并可发展为肝硬化［1］。肝纤维化是慢性肝病重要的病理特征，也是肝硬化发生的前奏和必经中间环节。阻断肝纤维化的发生和发展，对防治肝硬化和慢性肝病具有重要意义［2］。美国著名肝病学家Hans Popper指出:“谁能阻止或延缓肝纤维化的发生，谁将治愈大多数慢性肝病”。软肝颗粒(HG颗粒)是由丹参、桃仁等中药组成的复方制剂, 此方来源于