5 km高空缺氧对小鼠肝脾及血清中Zn ，Cu及Fe影响.doc

5 km高空缺氧对小鼠肝脾及血清中Zn ，Cu及Fe影响 　【摘要】 　　目的： 探讨5 km高空缺氧和不缺氧对小鼠肝、脾及血清中Zn, Cu, Fe的影响. 方法： 高空缺氧试验：将雄性昆明种小鼠20只，随机分为实验组(5 km高空缺氧30 min组)和地面对照组，每组10只. 将实验组小鼠置于低压舱内，以40 m/s的速度上升到5 km，停留30 min后，以40 m/s速度降至地面；地面对照组放置在另一低压舱中留在地面. 连续7 d后，抽取小鼠静脉血液并处死小鼠取肝、脾. 高空不缺氧试验：分组及实验方法同上. 舱内压力为54.02 kPa，其中氧分压为21.2 kPa. 舱内容积百分比为41.8%氧和58.2%氮的混合气体. 用测氧仪监测舱内氧压，保持其肺泡氧分压与地面相同. 采用火焰原子吸收法测定血清Zn，Cu和Fe的含量. 结果： 5 km 高空缺氧组与对照组相比较,小鼠肝中Cu和Fe含量均显著降低,差异有统计学意义(Plt;0.05)；脾中Fe的含量明显升高,差异有统计学意义(Plt;0.05)；两组血清中Zn，Cu和Fe含量均无明显变化,差异无统计学意义(Pgt;0.05). 5 km 高空不缺氧组与对照组相比较,小鼠肝、脾中Zn，Cu和Fe含量均降低,差异具有统计学意义(Plt;0.05),血清中Zn，Cu和Fe含量均无明显变化,差异无统计学意义(Pgt;0.05). 结论： 5 km高空缺氧时对小鼠肝中Cu和Fe的合成产生影响；5 km高空缺氧和不缺氧时小鼠血清中Zn，Cu和Fe的含量无明显变化，表明其含量可能与机体缺氧无关. 【关键词】 高海拔缺氧；肝；脾；血清；锌；铜；铁 【Abstract】 AIM: To explore the influence of 5 km altitude hypoxia and anoxia on Zn, Cu, Fe in mouse liver, spleen and serum. METHODS: Experiment 1: 20 male mice were divided into experimental and control group randomly(n=10). The experimental group was put into lowpressure module and ascended to 5 km altitude at the speed of 40 m/s and stayed for 30 min, then descended to the ground at the same speed. The control one was put into another lowpressure module on the ground, successively for 7 d, and then the mice were killed and livers, spleens and sera were taken. The contents of Zn, Cu and Fe were determined by the method of flame atom absorption. Experiment 2: The grouping and maneuvers were the same with the above experiment except that the pressure in the module was kept 54.02 kPa in total pressure and 21.2 kPa in partial pressure and the mixed gas comprised 41.8% oxygen and 58.2% nitrogen. The alveolar oxygen partial pressure was monitored by oxygen analyzer in module to keep the same with the one at ground. RESULTS: In experiment 1, compared with control group, the contents of Cu and Fe in liver were significantly decreased (Plt;0.05) and the Fe content in spleen was significantly increased (Plt;0.05) in the experimental group; the contents of Zn, Cu and Fe in bo