兔实验性肺栓塞后组织型纤溶酶原激活物抑制因子-1在肺动脉中动.docVIP

兔实验性肺栓塞后组织型纤溶酶原激活物抑制因子-1在肺动脉中动 　 作者：江爱桂，倪松石，卢慧宇，黄剑飞，姜声扬 【摘要】 目的：研究兔实验性肺血栓栓塞（PTE）后组织型纤溶酶原激活物抑制因子－1(PAI-1)在肺动脉中表达的动态变化。方法：日本健康大耳白兔48只随机平均分为栓塞组和对照组，栓塞组利用自体血栓经股静脉输入建立PTE模型，对照组输入生理盐水。用免疫组织化学及Western blot方法检测对照组及栓塞后即刻、4、8和24 h栓塞部位肺动脉组织中PAI-1蛋白表达的水平。结果：PAI-1在栓塞后即刻及24 h均见明显表达，而在栓塞后4 h和8 h表达明显减少；Western blot检测PAI-1蛋白在栓塞后4 h开始降低，8 h降低至低峰，与各对照组比较，差异有统计学意义(P lt;0.05)。结论：兔急性肺栓塞后，机体抑制纤溶能力表现为一过性降低，说明在肺动脉栓塞后，特别是严重的大面积栓塞后，单纯依靠肺动脉局部的纤溶抑制系统平衡变化不能达到溶解全部栓子的目的，其原因可能与PAI-1表达呈一过性下降有关。 【关键词】 肺栓塞；组织型纤溶酶原激活剂抑制物－1；肺动脉；免疫组织化学 SABC法；兔 [Abstract] Objective: To investigate the changes in plasminogen activator inhibitor-1(PAI-1) expression in pulmonary artery after acute pulmonary thromboembolism. Methods: Rabbit acute pulmonary thromboembolism models by injecting auto-blood clots into femoral vein were used to observe the protein expression of plasminogen activator inhibitor-1(PAI-1) in pulmonary artery. Control group received injection of the same volume saline. Specimens were obtained from both normal and morbid pulmonary artery 0、4、8 and 24 hours after PTE, and the expression of plasminogen activator inhibitor-1(PAI-1) was measured by immunohistochemistry and Western blot. Results: Immunohistochemical SABC method revealed PAI-1 staining in the endothelial cells of the morbid pulmonary artery 0 and 24 h after PTE but few in morbid pulmonary artery 4h and 8h after PTE. The staining data supported by Western blot data showed that the expression of the PAI-1 diminished progressively at 4 h and 8h after PTE(Plt;0.05). Conclusion： The study shows that the fibrinolysis inhibitory activity is decreased transiently after pulmonary thromnoembolism in rabbit, which indicate that it is not enough to dissolve the embolus just by the local fibrinolysis activity of pulmonary artery. [Key words] Pulmonary thromboembolism; Plasminogen activator inhibitor-1; Pulmonary artery; Immunohistochemical SABC method; Rabbit 组织型纤溶酶原激活剂（tissue plasminogen activator, t-PA）已经作为治疗肺栓塞一种安全有效的药物而广泛用于临床治疗[1]。纤溶酶原激活剂抑制因子-1（pla