忧虑康液对肾阴虚抑郁模型大鼠血浆促肾上腺皮质激素、皮质醇及下.docVIP

忧虑康液对肾阴虚抑郁模型大鼠血浆促肾上腺皮质激素、皮质醇及下 　 作者：陈晓阳 李晟 邹志 秦莉花 丁志高 蒋孟良 【摘要】 目的 探讨忧虑康液对抑郁肾阴虚模型大鼠血浆促肾上腺皮质激素(ACTH)、皮质醇(CORT)含量及下丘脑细胞形态学的影响。方法 采用慢性不可预见性应激并灌胃甲状腺素加孤养法复制抑郁肾阴虚大鼠模型，以放射免疫方法测定血浆ACTH、CORT水平，HE染色光镜下观察各组大鼠下丘脑形态学变化。结果 与正常组比较，抑郁肾阴虚模型大鼠血浆ACTH、CORT含量显著上升(Plt;0.01)，下丘脑形态学表现室旁核界限不清、神经元胞体增大、部分出现空泡样变性。与模型组比较，忧虑康液各剂量组及氟西汀组下调血浆ACTH、CORT浓度具有统计学意义(Plt;0.01，Plt;0.05);忧滤康液中、高剂量组可恢复抑郁大鼠下丘脑神经元数目，改善细胞形态，减少细胞空泡样变化，增大核/质比例。结论 慢性轻度不可预见性应激并灌胃甲状腺素加孤养法，可使大鼠神经内分泌发生异常改变，引起抑郁状态;忧虑康液可能通过降低血浆ACTH、CORT浓度及保护下丘脑结构而拮抗抑郁。 【关键词】 慢性应激;形态学;忧虑康液;大鼠;实验研究 　　【Abstract】 Objective To approach the effect of Youlükang on the ACTH，CORT density in the plasma and hypothalamus cell morphology of modeling rats following deficiency of kidneyYIN depressed.Methods Model of deficiency of kidneyYIN depressed rats were by using chronic unpredictable stress and oral thyroxine plus Custody Act alone method， radioimmunity determine was used to detected ACTH，CORT levels in the plasma, HE dyeing was given to observe every group rats′ hypothalamus morphologic changes.Results The ACTH，CORT density in the plasma of the deficiency of kidneyYIN depressed modeling rats were increased significantly, compared with normal group (Plt;0.01). The morphology of hypothalamus exhibited paraventricular with unclear bourn and neurons increased，even part of some place emerging vacuolesly degeneration.Compare with model group，You lü kang each dose group and the fluoxetine group reduced plasma ACTH，CORT concentration were statistically significant (Plt;0.01,Plt;0.05);and the number of neurons in the hypothalamus some recovery，neurons vacuoles degeneration could be improved.Conclusions Chronic light unpredictability and stress hormone administered alone raised increasing act can make in rat neuroendocrine abnormalities and cause depression;Youlükang shows a certain degree of antagonism by decreasing ACTH,CORT and protecting hypothalamus structure. 　　【Key words】 Chronic stress;Morphology;Youlükang;Rat;Experimental study 　　 忧虑康液系中药复方，具有滋阴填髓、解郁除烦之功效，该方