异丙酚、氯胺酮影响兔蓝斑去甲肾上腺素实验的研究.pdfVIP

rabbitonarabbit-tablelightlyandsoftly,caressedittomakeitcomfortable. TheLCwasperfusedwithartificialcerebrospinalfluidataconstantrateof O.lml/min.Thedrainagewascollectedintheenvironmentwithdarklight andicedbath.Beforetheinjectionofthestudieddrugs,weperfusedtheLC ofrabbitwithartificialcerebrospinalfluidfor20minutestomakeabalance, andthencollectedthecontrolspecimenofcerebrospinalfluidoftheawake animal.Inthefirstpartoftheexperiment,thepropofolof2mg/kgwas injectedintravenouslyin1minuteandfollowedbyaconstantinfusionof 150pg-kgl-minfor30minutes,wecollecteda specimenevery10minutes; Inthesecondpart,theketamineof2mg/kgwasgivenintravenouslyand followedbyaconstantinfusionof50，g-kg-min1for30minutes,we collectedaspecimenevery20minutes.TheconcentrationofNEinthe drainagesolutionwasmeasuredwithHPLC.Results:theconcentrationof NEinlocusceruleusdecreasedsignificantly(P0.01)afterthepropofol administration.Themaximumdecreasewas75.5%(rfom15.9士3.2pg/p1 to3.9土0.5pg/p1)，whichoccurredafterthebolusinjectionofpropofol. TheconcentrationofNEinlocusceruleusincreasedsignificantlyafter ketamineinjection(from16.013.Opg/p1to31.6士3.5pg/pI)(P0.01). Twentyminutesafterwithdrawingtheinfusionofstudiedmedications,the concentrationofNEinthetwogroupsrecoveredtothebaseline. Conclusion:PropofoldecreasestheconcentrationofNEinlocus ceruleus,whichmaybeitspossiblecentralmechanism ofcirculation inhibition.Ketamineni cre ases theconcentrationofNEinlocusceruleus, whichmaybeconsideredasapossiblecentralmechanismofitscirculation excitement. Keywords:propofol,ketamine,Push-pullperfusionofbrain,locus ceruleus,mradrenaline 3第一部分 异丙酚对兔蓝斑去甲肾上腺素的影响 3.1引 言 异丙酚是临床上广泛应用的全麻药，其最常见的主要不良反应是循 环抑制，表现为血压下降或伴有心率减慢。研究表明异丙酚引起的循环 抑制是多部位、多环节的综合作用。它可以通过影响心肌离子通道[[1,2]、 0肾上腺素受体一cAMP信号转导系统[[31及肌浆网钙稳态等因素4[[1引起对 心肌的直接抑制作用;同时，它可以通过对血管内皮细胞依赖的和非血 管内皮依赖的扩血管作用导致血压下降[5.61;此外，它还可能通过阻滞 交感神经节而引起扩血管作用[[7