miR-SNP及结直肠癌易感性及化疗疗效相关性的研究.pdfVIP

miR-SNP 与结直肠癌易感性及化疗疗效相关性研究 英文摘要 The association of miR-SNP with the susceptibility of colorectal cancer and response to chemotherapy Abstract Part I, The association of miR-SNP with the susceptibility of colorectal cancer and response to chemotherapy Objective: To investigate the correlation of the single nucleotide polymorphisms(SNPs) in the miRNA with the susceptibility, clinical features and response to chemotherapy of colorectal cancer. Methods: Firstly, we screened SNPs in the miRNA by using informatics softwares . Secondly, we collected peripheral blood of 300 healthy volunteers and 175 colorectal cancer patients and extracted DNA from them. Then, by allele-specific amplification method, we determined the genotype of the SNPs. Finally, we analyzed the correlation of SNPs with the susceptibility, clinical features and response to chemotherapy of colorectal cancer statistically. Results: (1) We analyzed 6 SNPs in the miRNA and found that the frequencies of alleles and genotypes (rs2910164, rs895819, rs7911488 and rs2292832) were significantly different between healthy controls and colorectal cancer patients (P0.05). Among them, rs7911488 and rs2292832 were the most important risk factors for developing colorectal cancer. Compared with the rs7911488 AA genotype, the individuals with AG genotype (OR=0.406, 95% CI=0.257-0.642) and G allele carriers (OR=0.469, 95% CI=0.300-0.732) had a lower risk of suffering from colorectal cancer significantly. Compared with the rs2292832 TT genotype, the individuals with CT genotype (OR=8.786, 95% CI=5.237-14.740) and C allele carriers (OR=4.