晚期非小细胞肺癌患者血清酸性谷胱甘肽S转移酶表达及临床意.docVIP

晚期非小细胞肺癌患者血清酸性谷胱甘肽S转移酶表达及临床意 　【摘要】 目的 通过测定非小细胞肺癌（Nonsmall cell lung cancer，NSCLC）患者血清中酸性谷胱甘肽S转移酶（GSTπ）的含量，探讨GSTπ在临床的应用价值。方法 应用ELISA法检测46例NSCLC， 19例肺良性病变者（Benign lung diseases，BLD）及60例健康人血清GSTπ含量，对46例NSCLC患者行诺维本加顺铂方案化疗两周期并判断疗效。结果 非小细胞肺癌患者血清中GSTπ含量明显高于肺良性病变者（Plt;0.05）及健康对照者（Plt;0.05）。以正常对照组(±2s，166.14ng/ml）为正常上限，NSCLC患者阳性率为54.34%，明显高于肺良性病变组36.84%（Plt;0.05）及正常对照组3.33%（Plt;0.05）。血清GSTπ含量在鳞癌中的阳性表达率为57.14%，腺癌为52.00%，两者间的差别无统计学意义（P＞0.05）。血清GSTπ表达与NSCLC组织学类型、分期、性别无关（Pgt;0.05）。46例接受了以顺铂为基础的化疗的NSCLC中，19例对化疗有效，总有效率41.30%。其中25例GSTπ表达阳性的NSCLC患者中5例对含铂方案化疗有效，有效率20%。21例GSTπ表达阴性的NSCLC患者中14例对含铂方案化疗有效，有效率66.67%。两组间化疗效果的差别有统计学意义（Plt;0.05）。结论 血清GSTπ可作为NSCLC的肿瘤标志物，血清GSTπ表达与NSCLC组织学类型、分期、性别无关。NSCLC患者血清中GSTπ的表达阳性者对含铂化疗方案的敏感性低于GSTπ的表达阴性者。GSTπ的含量是一个有价值的可预测患者对含铂化疗方案的敏感性的指标。 【关键词】 非小细胞肺癌；血清；谷胱甘肽S转移酶 　　Expression of the Serum GSTπ Level in Patients with Advanced NSCLC and Its Clinical Significance 　　HAN Zhigang, SHAN Li, LIU Chunling 　　Department of chemotherapy, Affiliated Tumor Hospital, Xingjiang,Wulumuqi 830011,China 　　Abstract：Objective To detect the expression of serum glutathione Stransferaseπ （GSTπ） level in patients with NSCLC,and to investigate its clinical significance.Methods GSTπ levels in serum of 46 patients with NSCLC, 19 patients with benign lung diseases and 60 health subjects were detected with ELISA. Fortysix NSCLC patients were given 2 cycles of NVB+DDP chemotherapy and clinical response was evaluated.Results The mean serum GSTπ level was significantly higher in patients with NSCLC than in those with benign lung disease and health subjects (Plt;0.05). Twentyfive of 46 patients with NSCLC (54.34%) showed elevated serum GSTπ levels above a cutoff value of 166.14ng/ml (mean+two standard deviations in 60 healthy control subjects). The positive rate was significantly higher in patients with NSCLC than in those with benign lung disease 36.84% and heavy subjects 3.33%(Plt;0.05).The positive rate of serum GSTπ were 57.14% in squamous cell carcinoma and