肺癌患者Th1-Th2细胞因子免疫反应状态变化及临床意义.docVIP

肺癌患者Th1/Th2细胞因子免疫反应状态变化及临床意义 　 作者：黄萍萍，唐德燊，吴平，陈捷，何惠娟，杨展 【摘要】 目的：分析肺癌患者外周血分泌不同细胞因子的T细胞数以及血清中6种细胞因子的水平，为肿瘤的免疫治疗提供实验依据。方法：先用刺激物刺激外周血T细胞，以增加细胞内细胞因子的表达，继用流式细胞仪分析特异性细胞因子表达水平。同时用酶联免疫吸附试验(ELISA)检测血清中相应的细胞因子量。结果：肺癌患者Th1型细胞因子干扰素γ(IFNγ)、白细胞介素2(IL2)、白细胞介素12(IL12)表达水平较正常对照组显著降低，Th2型细胞因子白细胞介素4(IL4)和白细胞介素10(IL10)表达水平较正常对照组升高，差异有显著性。肿瘤坏死因子a(TNFa)表达水平较正常对照组升高，差异有显著性意义。结论：肺癌患者体内Th2型细胞因子模式占优势状态，这可能是肿瘤细胞发生免疫逃逸的原因之一。 【关键词】 肺癌；细胞因子；辅助性T淋巴细胞亚群；流式细胞术 　　The Reaction State and Clinical Signficance of TH1/TH2 Cytokines in Lung Cancer 　　Abstract： Objective To analysed the amount of Tlymphocytessecreting different cytokines by peripheral blood and the serum level of those cytokines in the patients with lung cancer． In order to find out the laboratory evidence of tumor immunotherapy．Methods The peripheral blood lymphocytes were stimulated by Stimulator in order to enhance expression of cytokines . Those cytokines were detected by flow cytometry and enzyme linbed immunosorbent assay(ELISA)． Results The level of Th1 type cytokines such as interferonγ(IFNγ),intreleukin2(IL2)，interleukin12(IL12) were significantly lower in patients with lung cancer than those normal controls． The levels of Th2 type cytokines such as interleukiin4(IL4)，interleukin10(IL10)were higher in pstients with lung cancer than those in normal controls,but there were not significant difference between them.The level of tumor necrosis factor a(TNFa)in patients were significant higher than that in normal control. Conclusion Th2 type cytokines are dominant in lung cancer.It maybe one of the causes of immune escape. 　　Key words: Lung cancer;Cytokines;Th1/Th2 subsets;Flow cytometry 　　辅助性T细胞(Th)对机体免疫系统有重要的调节作用。人在正常的生理情况下，Th1、Th2两类细胞处于相互抑制、相互转化的平衡状态，一旦平衡被打破，机体就会处于Th1优势或Th2优势的漂移状态，从而出现各种病理反应，甚至发生肿瘤［1，2］。近年来的研究发现，多数肿瘤患者体内呈现Th2优势的漂移状态［3～5］，并且肿瘤本身也可分泌多种细胞因子，影响肿瘤的微环境，因此肿瘤患者体内Th1/Th2状态与肿瘤发生、发展及预后密切相关。我们应用流式细胞仪通过CytodetectTM试剂盒检测肺癌患者CD3+T细胞分泌细胞因子的表达，用酶联免疫吸附试验(ELISA)法检测血清中相应的细胞因子水平，分析患者的Th1/Th2免疫反应状态的变化，现结果报道如下。 　　1 对象和