银黄方膜分离物红外表征及药效组分含量关系分析.docVIP

银黄方膜分离物红外表征及药效组分含量关系分析 　【摘要】 目的 研究银黄方水煎液多极膜分离物的红外指纹表征和药效成分含量之间的关系，为建立FT-IR对多极膜分离过程中的在线质量控制提供科学数据。方法 采用傅里叶变换红外光谱（FT-IR）法和高效液相（HPLC）法，分析和比较银黄方水煎液、各个膜分离产物红外指纹表征及其相关系数；测定有效成分黄芩苷、绿原酸的含量，计算收率。结果 银黄方水煎液的红外特征指纹峰有29个 （νcm-1），超滤透析液红外特征指纹峰有34个（νcm-1），超滤截留液红外特征指纹峰有22个（νcm-1），纳滤截留液红外特征指纹峰有25个（νcm-1），纳滤透析液红外特征指纹峰有26个（νcm-1）。各膜分离物与水煎液的相关系数分别为0.9001、0.5436、0.8327、0.1128。所得膜分离产物以超滤膜透析液和纳滤膜截留液药效组分转移率最高，分别为黄芩苷87.65%和84.12%；绿原酸为92.90%和89.68%。结论 通过红外分析和含量测定可见在红外特征峰一致以及相关系数较高的膜分离物中，药效组分（黄芩苷和绿原酸）的含量亦较高。本研究方法的建立，可以对复方中药膜分离的在线质量控制提供科学数据。 【关键词】 FT-IR指纹 膜分离 银黄 【Abstract】 Objective To study the relation between the FT-IR fingerprint characteristics and the content of active ingredient on Yinhuang medicin broth and its membrane separation fraction.Methods We adopt FT-IR,the second order derivative and HPLC ,to analysis and compare the FT-IR fingerprint characteristics and the related ratio of Yinhuang medicinal broth and its membrane separation fraction; To detection the content of baicalin and caffeotannic acid and to calculate receipt rate.Results The FT-IR fingerprint characteristic that Yinhuang medicinal broth has twenty-nine peaks; that ultrafiltration dialysate liquid has thirty-four peaks; that ultrafiltration entrapment liquid has twenty-two peaks; that nanofiltration dialysate liquid has twenty-six peaks; that nanofiltration entrapment liquid has twenty-five peaks. the coefficient correlation of Yinhuang medicinal broth to each membrane separation fraction is 0.9001、0.5436、0.1128、0.8327 in order. In the membrane separation fraction, effective constituents diversion rate of ultrafiltration dialysate liquid and nanofiltration entrapment liquid is highest. Baicalin is 87.65% and 84.12% in order; caffeotannic acid is 92.90% and 89.68% in order.Conclusion In membrane separation fraction, when the infrared feature is conformity and coefficient correlation is higher,the content of effective constituents（baicalin and caffeotannic acid）is also higher.The establishment of this research techni