专注创新诚信和谐.PDFVIP

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2017-09-24 发布于上海
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专注创新诚信和谐.PDF

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百恩维生物科技有限公司专注创新诚信和谐专注创新诚信和谐仅供参考深圳市百恩维生物科技有限公司地址：深圳市宝安区67 区留仙一路高新奇战略新兴产业园二期1 号楼5 楼邮政编码： 518100 电话： + 86 - 755 - 传真： + 86 - 755 - 网址：百恩维生物科技有限公司目录一、慢病毒载体系统 ·························································································3 二、慢病毒的优点 ····························································································4 三、293T （Biowit 货号：C0002）细胞培养···························································4 1. 293T 细胞培养特性··················································································4 2. 293T 细胞完全培养液···············································································5 3. 293T 细胞的复苏·····················································································5 4. 293T 细胞的传代·····················································································5 5. 293T 细胞的冻存·····················································································6 四、慢病毒包装·······························································································7 1. HET(Biowit 产品号：BW11002)转染293T 细胞 ·············································7 五、慢病毒滴度测定（荧光法）··········································································8 六、慢病毒感染目的细胞预实验··········································································9 七、慢病毒的储存、稀释 ··················································································10 八、使用安全注意事项 ·····················································································11 九、慢病毒操作注意事项··················································································11 附录··········································································