冠心病人单核细胞ABCA1基因表达及非诺贝特对ABCA1表达影响.pdfVIP

The Expression of ABCA1 in Monocytes of Patients with Coronary Heart Disease and Effects of Fenobrate on the Expression of ABCA1 ABSTRACT Objective To investigate the relationship between the degree of cornary atherosclerosis and the expression of ATP binding cassette transporter A1(ABCA1) and the effects of fenobrate on the expression of ABCA1 in monocytes of coronary heart disease(CHD) patients. Methods People who underwent coronary angiography were divided into different groups according to the severity of cornary sterosis.Serum lipids were measured in all subjects.Monocytes were isolated by density gradient method from blood of samples.Total RNA of monocytes were abstracted and reverse transcription polymerase chain reaction (RT-PCR) were performed to inspect the expression of ABCA1 mRNA in different groups.Western blot were performed to inspect the expression of ABCA1 protein level in different groups.Monocytes in CHD groups were incubated with fenobrate. The mRNA and protein level of PPARα and ABCA1 were inspected. Results There were stastistic difference at the level of TC、LDL-c、 HDL-c in control group and CHD groups(P0.05). There were no stastistic 5 difference at the expression of ABCA1 mRNA and protein in control group and CHD groups(P0.05). Fenobrate can increase mRNA level of PPARα and ABCA1 in monocytes of CHD patients (P0.05).Fenobrate can upgraduate the protein expression of PPARα and ABCA1 in monocytes of CHD patients (P0.05). Conclusions The increased level of HDL-c and the decreased level of TC and LDL-c were correlated with the s