2007年基因打靶技术_开启遗传学新纪元.pdf

HEREDITAS (Beijing) 2007 11 , 29(11): ISSN 0253-9772 DOI: 10.1360/yc-007-1291 , , 100071 : , : ; ; ; Gene targeting: the beginning of a new era in genetics TENG Yan, YANG Xiao Genetic Laboratory of Development and Diseases, Beijing Institute of Biotechnology, Beijing 100071, China Abstract: Gene targeting, which allows the efficient modification of specific murine genes, plays important roles in eluci- dating physiological function of genes in vivo, dissecting the genetic mechanisms of human disease and identifying poten- tial targets for drug development. In recent years, the development of conditional gene targeting has provided novel oppor- tunities for gene inactivation in specific cells or organs, and at specific time points, both during development and in the adult animals. In this review, we introduce the history of gene targeting and recent progress of gene targeting technology and its applications in other model organisms. Keywords: gene targeting; mouse; conditional gene targeting; gene knockout 20 , , 2007 10 8 , Mario R. Capecchi Oliver Smithies [1] Martin J. Evans 1 2007 [2] , 2007- 10-16; 2007-10-19 (973 )(2005CB522506; 2006CB943501) (863 )(2006AA02Z168) (2006BAI23B01-3)( Z0006303041231)[Supported by National Basic Research Program of China(973 Program) (No.2005CB522506; 2006CB943501), Chinese National Natural Science Foundation (No, Hi-Tech Rese