高分辨率熔解技术检测冠心病患者TNF-α及其受体基因多态性.pdfVIP

JClinLab ·405· 临床检验杂志2014年6月第32卷第6期ChinSci，Jun．2014，V01．32，No．6 ·临床检验技术研究· DOI：10．13602／j．cnki．jcls．2014．06．02 高分辨率熔解技术检测冠心病患者TNF—o／．及其受体 基因多态性水 化系肿瘤重点实验室，兰州730030) 摘要：目的 (SNP)，探讨其与冠心病(CHD)易感性及血脂指标的相关性。方法 TNFA一863CA、姗J-383AC和TNFR2．exonTG 州地区汉族人群CHD易感性相关，TNFR2一exonTG位点与CHD患者HDL—C水平相关联。 关键词：高分辨率熔解技术；肿瘤坏死因子ot；单核苷酸多态性；冠心病 中图分类号：R541．4 文献标志码：A Genetic of andits detected resolution inthe with TNF·a polymorphisms receptorgenes byhigh meltingtechnologypatients disease coronaryartery QIXiao—min91，ZHANGRuil，LIQian·Mnl，￡UMing—hurtl，YOUChong—gel’2(1．Center’forLaboratoryMedicine，LanzhouUniversity Second 730030，Gansu；2．Gansu Tumor，Lanzhou Hospital，Lanzhou KeyLaboratoryofDigestiveSystem To theassociationofthe nucleotide TNF-otandits de— Abstract：Objective investigate single polymorphism(SNP)ofreceptorgenes tected resolution withthe of heart serum byhigh melting(HRM)technologysusceptibilitycoronarydisease(CHD)andcholesterol，tri— andLDLlevelsinHan of Lanzhou ThePCR-HRMwasestablishedandusedto aeylglyeerol，HDL population region．Methods assay detectsixSNPsofTNF一0【andits receptorgenes，including TNFRl—383ACandTNFR2一exonTG．in207CHD and274 controls．Then．thecorrelationsofthesixSNPswith patients healthy ofCHDandserum and the LDLlevelswere thealleleand susceptibility cholesterol，triacylglycerol，HDLanalyzed．ResultsOnly ofTNFA一863CAwere differentbetweenCHD andthe and genot