信号传导和转录激活因子3及其蛋白抑制剂在狼疮-南方医科大学学报.pdfVIP

2012;32(6) 南方医科大学学报（J South Med Univ ） ·821 · 基础研究 信号传导和转录激活因子3 及其蛋白抑制剂在狼疮小鼠肾组织 中的表达 杨 荟，徐安平，吕 军，刘 亚 中山大学孙逸仙纪念医院肾内科，广东 广州 510120 摘要：目的探讨信号传导和转录激活因子3(STAT3)及其蛋白抑制剂(PIAS3)在MRL/lpr 狼疮小鼠肾组织中的表达。方法以18 周龄雌性MRL/lpr 小鼠作为狼疮肾炎组，相同周龄的BALB/C 小鼠作为正常对照组。分别取两组小鼠肾组织，应用荧光定量 RT-PCR 法检测STAT3 和PIAS3 mRNA 的表达，采用Western blot 和免疫组织化学方法检测磷酸化STAT3（p-STAT3）和STAT3 的蛋白表达，同时检测小鼠实验室指标以及肾脏组织病理的表现。 结果MRL/lpr 小鼠尿蛋白、血尿素氮、血肌酐均高于BALB/ C 小鼠（P0.05）。MRL/lpr 小鼠肾脏组织病理主要表现为肾小球系膜细胞增生，肾小管间质炎症细胞浸润，而BALB/C 小鼠肾 小球、肾小管无异常。MRL/lpr 小鼠肾组织STAT3 mRNA 和p-STAT3 蛋白的表达均较BALB/C 小鼠升高(P0.05) ，其PIAS3 mRNA 表达则较BALB/C 小鼠明显降低(P0.01) ，而两组小鼠STAT3 总蛋白的表达无明显差别。结论MRL/lpr 小鼠肾组织 STAT3 mRNA 和p-STAT3蛋白的表达均高于对照组，而其PIAS3 mRNA 表达低于对照组。 关键词：STAT3转录因子；活化STAT 的蛋白抑制物；信号传导；红斑狼疮，系统性；狼疮肾炎 中图分类号：R586 文献标志码：A 文章编号：1673-4254（2012）06-0821-05 doi: 10.3969/j.issn. 1673-4254.2012.06.014 /kcms/detail/44.1627.R1054.001.html Expression of STAT3 and PIAS3 in renal tissues of MRL/lpr mice YANG Hui, XU Anping, LÜJun, LIU Ya Department of Nephrology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China Abstract: Objective To investigate the expression of STAT3 and PIAS3 in the renal tissues of rats with lupus nephritis. Methods The kidneys were harvested from 18-week-old female MRL/lpr mice (lupus nephritis model group) and age-matched female BALB/C mice (normal control group). The expressions of STAT3 and PIAS3 mRNA in the renal tissues were measured by real-time quantitative RT-PCR, and the protein levels of STAT3 and p-STAT3 were examined using Western blotting and immunohistochemistry. The laboratory indices and renal histopathology of the mice were also investigated. Results The urinary protein, blood urea nitrogen (BUN) and creatinine levels were significantly higher in MRL/lpr mice than in the cont