赖氨大黄酸对hela细胞周期的影响及其机制-中国现代应用药学.pdfVIP

·论 著· 赖氨大黄酸对 HeLa 细胞周期的影响及其机制 1 2 1 1* 林雅军 ，甄永占 ，魏洁 ，胡刚 (1.卫生部北京医院/卫生部北京老年医学研究所，北京 100730 ；2.华北煤炭医学院，河北 唐山 063000) 摘要：目的 探讨赖氨大黄酸对 HeLa 细胞周期的影响及其机制。方法 MTT 法检测赖氨大黄酸对HeLa 细胞增殖的影 响；流式细胞技术检测活性氧水平和细胞周期变化；显微镜下观察HeLa 细胞形态变化；Western blot 法检测p53 和p21 蛋白表达。结果 赖氨大黄酸剂量依赖性地抑制HeLa 细胞增殖，IC50 约为 83 µmol·L1。随着赖氨大黄酸剂量的增加， HeLa 细胞中活性氧水平增加，并出现S 期和G2 期阻滞，以及细胞空泡样变性。通过Western blot 研究发现赖氨大黄酸 处理后的p53 蛋白磷酸化水平呈剂量依赖性地增加，同时下游周期相关蛋白p21 表达水平也随之增加，而p53 蛋白表达 水平未出现显著地剂量依赖性变化。结论 赖氨大黄酸通过增加 HeLa 细胞中活性氧水平，引起细胞周期相关蛋白 p53 的磷酸化水平和p21 蛋白表达水平增加，引起HeLa 细胞出现空泡样变性，并诱导S 期和G2 期阻滞。 关键词：HeLa 细胞；赖氨大黄酸；活性氧；细胞周期 中图分类号：R965.1 文献标志码：A 文章编号：1007-7693(2011)06-0489-05 Effect of Rhein Lysinate on Cell Cycle of HeLa Cell and Its Mechanism 1 2 1 1* LIN Yajun , ZHEN Yongzhan , WEI Jie , HU Gang (1.Beijing Geriatric Institute, Beijing Hospital, Beijing 100730, China; 2.North China Coal Medical College, Tangshan 063000, China) ABSTRACT: OBJECTIVE To explore the effect of Rhein lysinate on cell cycle of HeLa cells and its mechanism. METHODS The effect of Rhein lysinate on cell proliferation was detected by MTT. The level of reactive oxygen species and the change of cell cycle of HeLa cells were investigated by flow cytometry. Cell morphology was observed by microscope. The expression of p53 and p21 was determined by Western blot. RESULTS Rhein lysinate could inhibit the proliferation of HeLa cells in a dose-dependent manner. The IC50 was approximately 83 µmol·L1. The level of reactive oxygen species and the ratios of S period and G2 period cells were increased following the increase of Rhein lysinate. Moreover vacuolar degeneration was induced with the increasing of Rhein lysinate. The phosphorylation of p53 and the