花生白藜芦醇合成酶基因pnrs1的克隆及其在原核中的表达-作物学报.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(2): 341−346 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.00341 花生白藜芦醇合成酶基因PNRS1 的克隆及其在原核中的表达 韩晶晶1,2 刘 炜1,* 毕玉平1,2 1 / , 250100; 2 , 250014 : RT-PCR (resveratrol synthetic enzyme, RS), PNRS1 , GenBank FM955393 1 170 bp, 389 14 DNA PCR , 1 537 bp, 2 1 , PNRS1 RS 91%~95%, PNRS1 , UV-B PNRS1 pET-28a(+), IPTG 46 kD PNRS1 RS , : ; ; ; ; Molecular Cloning of Peanut Resveratrol Synthetic Enzyme 1 (PNRS1) and its Expression in Prokaryote HAN Jing-Jing1,2, LIU Wei1,*, and BI Yu-Ping1,2 1 Hi-Tech Research Center, Shandong Academy of Agricultural Sciences / Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Ji’nan 250100 China; 2 College of Life Sciences, Shandong Normal University, Ji’nan 250014, China Abstract: Resveratrol synthetic enzyme (RS) is a key and necessary enzyme that plays important role in resveratrol synthesis pathway. To uncover and characterize the function of the RS in plant developmental process, we isolated a RS gene PNRS1 (FM955393) by RT-PCR using total RNA of peanut seed as template, and the gene PNRS1 was expressed in E. coli prokaryote for further analysis. The results showed that the PNRS1 had a 1 170 bp open reading frame encoding 389 amino acid polypeptide, and exhibiting high similarities with other members of RS genes family. Expression pattern analysis indicated that the PNRS1 was spe- cially expressed in peanut root, and could be indu