超声波法提取猪血液中血红蛋白的研究 study on ultrasonic extraction of hemoglobin from pig red blood cells.pdfVIP

92 2009．V01．30,No．04 良晶科宇 ※工艺技术 超声波法提取猪血液巾血红蛋白的研究 周玲1，阚建全2，汪学荣t，· (1．西南大学荣昌校区动物科学系，重庆402460；2．西南大学食品科学学院，重庆400716) 摘 要：本实验研究了超声波处理过程中水与红细胞体积比、超声功率、超声时间与问歇时间比以及工作时问 等四个因素对血红蛋白提取率的影响，首先通过单因素试验初步确定各因素的范围，然后通过正交试验得出其最 佳提取工艺参数。结果表明：水与红细胞体积比为4：l，超声功率为100W，超声时问与间歇时间比为l：l，工 作时间为20min，血红蛋白提取率可达97．65％。 关键词：猪血；血红蛋白；超声波；提取 OnUltrasonicExtractionof fromRedBloodCells Study HemoglobinPig ZHOU Lin91。KANJian—quan2，WANGXue-rong’·’ ofAnimal School (1．DepartmentScience，RongchangDistrict，SouthwestUniversity，Chongqing402460，China； ofFood 2．College Science，Southwest 400716，China) University，Chongqing Abstract：SodiumcitrateWaSaddedintofreshbloodto thenthe blood blood，and pig prepareanticoagulant anticoagulant to was toobtainredbloodcells．Afterwithnormalsaline3times，theredbloodcellswereusedextract centrifugated washing mea／岱ofultrasonic．Effects blood ofultrasonictime tored hemoglobinby ofratio(v／v)ofwatercell，ultrasonicpower,ratio timeontheextractionrateof were factor interval，and test, 幻quiescent operation hemoglobininvestigated．Throughsingle their were thentheextraction were test． scopes determ呲and technologypararnctcrsoptimizedthroughorthogonal initially Theresultsshowedthatwhenthe watertoredbloodcellis ultrasonicis10