毛脚鵟细胞体外培养体系建立及三种组织来源细胞特性分析.pdfVIP

动 物 学 研 究 2013，Jun. 34(3): 244−248 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI:10.11813/j.issn.0254-5853.2013.3.0244 毛脚鵟细胞体外培养体系建立及三种组织来源细胞特性分析 刘 刚，道日娜，王蓉蓉，李云霞，戴雁峰，李喜和，李 煜，李 瑶* 内蒙古大学 蒙古高原动物遗传资源研究中心，内蒙古 呼和浩特 010021 摘要：用组织块培养法对毛脚鵟不同组织进行原代培养，获得了3 种不同组织来源的细胞，并成功对细胞进行了冷冻保存和 复苏。在传代培养过程中，对比分析了 3 种组织来源细胞的形态学、生长曲线、贴壁率、核型等生物学特性。形态学方面， 3 种来源细胞均为成纤维样细胞。对于 3 种组织来源细胞的贴壁能力分析显示，输卵管源细胞最强，肺源细胞和气管源细胞 次之。3 种不同组织来源细胞的倍增时间分别为(29.91 ±0.39)、(33.18 ±0.21)和(30.67 ±0.28) h，群体倍增次数分别为 3.54 ± 0.01、4.52 ±0.02 和 4.38 ±0.03 。毛脚鵟细胞的染色体数目为 2n=68 ，性染色体为典型的 ZW 型。本实验为今后毛脚鵟细胞 利用、遗传信息的保存及生物学特性的深入研究提供实验材料和依据。 关键词：毛脚鵟；细胞培养；生长曲线；细胞贴壁率；核型分析 中图分类号：Q959.7+24; Q253 文献标志码：A 文章编号：0254-5853-(2013)03-0244-05 Establishment of cell culture system of rough-legged buzzard and biological characteristic analysis on different tissue cells cultured in vitro * Gang LIU, Daorina, Rong-Rong WANG, Yun-Xia LI, Yan-Feng DAI, Xi-He LI, Yu LI, Yao LI Institute of Animal Genetic Resources of Mongolia Plateau Inner Mongolia University, Huhhot 010021, China Abstract: In total, three different tissues from the rough-legged buzzard were obtained by culture and successfully cryopreserved and then recovered. During the subculture process, biological characteristics including as cell morphology, growth curve, cell adhesion rate, and karyotype were analyzed and compared, and overall all three kinds of tissue cells exhibited fibroblast-like growth. Oviduct-derived cells had the strongest adherent ability, followed by lung-derived cells and trachea-derived cells. The doubling times of lung-derived cells, trachea-derived cells, and oviduct-derived cells were 29.91±0.39 h, 33.18±0.21 h, and 30.