抑制自噬促进贝伐珠单抗对结肠癌细胞的生长抑制和凋亡诱导.pdfVIP

现代肿瘤医学 2017 年 02 月 第 25 卷第 03 期 MODERN ONCOLOGY ，Feb . 2017, VOL . 25, NO . 03 • 345 • 抑制自噬促进贝伐珠单抗对结肠癌细胞的生长抑制和凋亡诱导 钟 滴 ，赵 志 Inhibition of autophagy promote Bevacizumab - induced growth inhibition and apoptosis of colon cancer Zhong L i, Zhao Zhi Gastrointestinal Department ^the Affiliated Hospital o f Guilin Medical College ^Guangxi Guilin 541QQQ, China. 【 Abstract 】 Objective : To explore the relationship between anti - VEGF and autophagy. Methods ： After HT - 29 cells treated with Bevacizumab in the absence or presence of CQ,we explored apoptosis,proliferation and autophagy. Results VEGF ： were upregulated in colorectal cancer. Moreover, patients with high VEGF expression showed signifi­ cantly shorter overall survival. In addition, Bevacizumab inhibited the viability of human colon cancer cells in a dose - and time - dependent manner and induce apoptosis. At the last, treatment with Bevacizumab induced protective auto­ phagy in colon cancer cells. Conclusion Inhibition of autophagy promotes ： the effect of growth inhibition and apoptosis of colon cancer. 【 Key words 】anti - VEGF ，Bevacizumab autophagy，， colon cancer Modem Oncology 2 01 7,2 5(03) ：0345 -0351 【摘要】 目的:探讨抑制肿瘤血管内皮生长因子和自噬的相互关系 。方法:利用贝伐珠单抗和（或）自噬抑制 剂氯喹与结肠癌细胞HT - 2 9 共同孵育后，检测其生长抑制，凋亡及自噬发生的情况。结果:在人结直肠癌石 蜡切片中,V E G F 的表达明显增高，并且表达高的患者预后较差。利用贝伐珠单抗可以抑制结肠细胞的增殖、 凋亡以及诱导细胞保护性自噬的发生。利用氯喹抑制自噬后，能够促进贝伐珠单抗对细胞的增殖抑制和凋 亡诱导 。结论:抑制自噬能够促进贝伐珠单抗对结肠癌细胞的生长抑制和凋亡诱导。 【关键词】抗VEGF; 贝伐珠单抗；自噬;结肠癌 【中图分类号】R735. 3 + 5 【文献标识码】 A D O I ：10. 3969/j. issn. 1672 -49 9 2 . 2017. 03. 003 【文章编号】 1672 -49 9 2 - (2017)03 -0345 -0 7 据统计，结直肠癌在男性肿瘤的发病率中占据第三位， VEGF耐药的现象。因此，目