河南不同类型麦区小麦丰产高效栽培技术规程-河南农业科学.pdfVIP

D I :10.15933/j.cnki.1004 -3268.2006.05.003 2006 年第5期 王永华,郭天财,朱云集, 王晨阳,康国章 (, 450002) :介绍了河南省高产灌区、中产灌区和旱作区三大不同产量水平、生产条件和生态类型麦区小 麦丰产高效 培技术规程,着重阐述了其适宜区域、不同生育时期主攻目标、主要技术经济指标及 主要技术措施等。 :小麦;生态类型;丰产高效; 培技术规程 :S512.1　　:B　　:1004-3268(2006)05-0012-05 　　, 。 , 、 ,、、、、, , , , 。, , 、, 、、 、 :2005-11-17 :(2004BA520A06) :(1973-), , , , 。 [28] 　Thomas H S, Laurie J J, Richard P H, et al .SYPR or- Protein reverse staining:high-eciency micro-yanalysis of ange and SYPR red protein gel stains:one-step fluo- unmodied proteins detected on electrophoresis gels[J]. rescent staining of denaturing gels for detection of Anal Biochem, 1995, 224:203-211. nanogram levels of protein[J] .Analytical Biochemistry, [35] 　Fernández-Patrón C, MadrazoJ, Hardy E, et a l.Single 1996, 239:223-237. -step electrotransfer of reverse-stained proteins from [29]　Hames B D, Rickwood D.In:Gel Electrophoresis of Pro- sodium dodecyl sulfate-poly-acrylamyde gel onto re- teins.A practical Approach, 2nd ed.[ M] .New York: verse-phase minicartridge and subsequent desalting and IRL Press, 1990.52-81. elution with a conventional high-performance liq-uid [30]　Gopal G, Thallampuranam K S K, Shanmugiah T K P, chromatography gradient system for analysis[J].Elec- et a l.Rapid staining of proteins on polyacrylamide gels trophoresis, 1995, 16:911-920. and nitrocellulose membranes using a mixture of fluores- [36] 　Castellanos-Serra L, Fernández-Patrón C, Hardy E, et cent dyes[J] .J Biochem Biophys Methods, 2000, 46:31 a l.A procedure for protein elution from reverse stained -38. polyacryl-amide gels applicable at the low picomole lev-