表没食子儿茶素没食子酸酯增强低剂量顺铂对小细胞肺癌H446细胞株的细胞毒作用.docVIP

表没食子儿茶素没食子酸酯增强低剂量顺铂对小细胞肺癌H446细胞株的细胞毒作用 赵汉玺1，胡旭东2，谢 鹏2，孙新东2，于金明2(250012 济南，山东大学医学院研究生院1；250117 济南，山东省肿瘤医院放疗四病区2) [摘要] 目的 探讨表没食子儿茶素没食子酸酯((-)-epigallocatechin-3-gallate, EGCG)联合顺铂（Cisplatin，DDP）对小细胞肺癌细胞株H446的细胞毒作用及其机制。方法 采用MTT法检测EGCG及DDP影响H446细胞增殖的量效和时效关系。采用流式细胞仪检测不同浓度EGCG、DDP及二者联合作用于H446细胞对细胞周期及凋亡的影响。结果 EGCG和DDP均可抑制H446细胞增殖，该增殖抑制作用呈浓度及时间依赖模式。不同浓度的EGCG与DDP联合应用时，可H446细胞阻滞于G2/M期G2/M期R734.2；R730.53；R73-36+1 [文献标志码] A (-)-epigallocatechin-3-gallate enhance low dose Cisplatin Cytotoxicity in human small cell lung cancer cell line H446 Zhao Hanxi1, Hu Xudong2, Xie Peng2, Sun Xindong2,Yu Jinming2(Department of postgraduate of Shandong Univeristy School of Medicine,Jinan, 2500121; Department of Radiotherapy Ⅳ, Shandong Tumor Hospital ,Jinan,2501172) [Abstract] Objective To investigate the effect of EGCG and Cisplatin (DDP) on cytotoxicity, apoptosis, and cell cycle in human small cell lung cancer cell line H446 cells. Methods Human small cell lung cancer cell line H446 cells were treated with different concentrations of EGCG and DDP; the proliferation of cells was assayed by MTT method; flow cytometry (FCM) was used to detect cell cycle arrest; cells apoptosis was analyzed by flow cytometry with Annexin V/PI staining. Results Treatment of EGCG led to a significant inhibition proliferation of H446 cells in a dose and time dependent manner. The percentage of cells in G2/M phases increased markedly after treatment with EGCG and low dose DDP. The apoptosis of H446 cells could be induced by EGCG and low dose DDP, the apoptosis rates was higher in the group of EGCG combined with DDP than either of them. Conclusion EGCG can effectively inhibit the proliferation of H446 cells and induce cell apoptosis; moreover, EGCG can enhance H446 apoptosis induced by low dose of DDP; the underlying mechanism may be due to the cell cycle arrest in G2/ M phases. [Key words] small cell lung cancer; EGCG; Cisplatin; apoptosis Corresponging author: Yu Jinming,Tel:86-531 E-m