表没食子儿茶素没食子酸酯增强低剂量顺铂诱导H446细胞凋亡的实验研究.doc

表没食子儿茶素没食子酸酯增强低剂量顺铂诱导H446细胞凋亡的实验研究 赵汉玺1，胡旭东2，谢鹏2，孙新东2，于金明2(250012 济南，山东大学医学院1；山东省肿瘤医院放疗四病区2) [摘要与DDP联合应用时，可H446细胞阻滞于G2/M期G2/M期R734.2；R730.53；R73-36+1 EGCG enhance the apoptosis induced by low dose of DDP in human small cell lung cancer cell line H446 Zhao Hanxi1, Hu Xudong2, Xie Peng2, Sun Xindong2,Yu Jinming2(Shandong Univeristy School of Medicine 44#,Wenhua Xi Road,Jinan,Shandong, 250012 P.R. China1, Department of Radiotherapy Ⅳ, Shandong Tumor Hospital, 440#,Jiyan Road,Jinan, 250117 P.R. China2) [Abstract] Objective To investigate the effect of EGCG and cisplatin (DDP) on the induction of apoptosis and regulation of cell cycle in human small cell lung cancer cell line H446 cells. Methods Human small cell lung cancer cell line H446 cells were treated with different concentrations of EGCG and DDP; the proliferation of cells was assayed by MTT method; flow cytometry (FCM) was used to detect cell cycle arrest; cells apoptosis was analyzed by flow cytometry with Annexin V/PI staining. Results Treatment of EGCG led to a significant inhibition proliferation of H446 cells in a dose and time dependent manner. The percentage of cells in G2/M phases increased markedly after treatment with EGCG and low dose [通信作者] 于金明, 电话:(0531 E-mail: 171459576@ DDP. The apoptosis of H446 cells was induced by EGCG, the apoptosis rates was higher in the group of EGCG combined with DDP than either of them. Conclusion EGCG can effective inhibit the proliferation of H446 cells and induce cell apoptosis; moreover, EGCG can enhance H446 apoptosis induced by low dose of DDP; The underlying mechanism may be due to the cell cycle arrest in G2/ M phases. [Key words] small cell lung cancer; EGCG; cisplatin; apoptosis 小细胞肺癌发病率占全部肺癌的16%。一般来说，与非小细胞肺癌相比，小细胞肺癌倍增时间短，生长分数高，易发生早期转移[1]。茶多酚主要成分为表没食子儿茶素没食子酸酯 ((-)-epigallocatechin-3-gallate，EGCG) ，有研究报道EGCG 对前列腺癌[2] 、白血病[3] 、鼻咽癌[4] 、大肠癌[5] 和宫颈癌[6]等肿瘤细胞均有抑制作用。研究还证实EGCG对敏感及耐药的SCLC细胞株也有抑制作用[7]，但目前尚未见EGCG与化疗药物联合应用于小细胞肺癌的体外实验研究。 因此，本研究观察EGCG和顺铂(c