调控RhoARac平衡对失血性休克大鼠血管双相反应性的影响.docVIP

调控RhoA/Rac平衡对失血性休克大鼠血管反应性的影响 400042重庆，第三军医大学附属大坪医院野战外科研究所，创伤、烧伤与 复合伤国家重点实验室 【摘 要】:目 的 观察调控RhoA/Rac平衡休克后血管反应性变化。方 法RhoA，Rac激动剂或抑制剂，观察其对休克早期或休克晚期大鼠平均动脉血压(mean arterial pressure， MAP)、肠系膜上动脉血管管径对NE收缩反应性的影响。ac激动剂PDGF和RhoA特异性抑制剂C3enzyme可降低休克早期肠系膜动脉对NE收缩反应性和NE升压反应性；RhoA激动剂U-46619和Rac特异性抑制剂NSC23766可改善休克晚期肠系膜上动脉对NE的收缩反应和NE的升压效果，也进一步升高休克早期收缩反应性； RhoA激动剂U-46619和Rac激动剂PDGF的作用可以分别被其特异性抑制剂所拮抗。结 论 用RhoA或Rac激动剂或拮抗剂可调控失血性休克大鼠血管反应性双相变化，提示休克早期RhoA活性升高，Rac活性降低，而休克晚期RhoA活性降低，Rac活性升高，RhoA/Rac活性平衡参与了休克后血管反应性双相变化的调节。 【关键词】: 休克；RhoA；Rac；血管反应性 The effects of regulation RhoA/Rac balance on biphasic vascular reactivity after hemorrhagic shock in rats LI Tao, XU Jing, LIU YANG Guang-ming Fang Xiao-qin, Liao Zi-fu, Chen Feng, Liang-ming（State Key Laboratory of Trauma, Burns and Combined Injury, Department 2, Research Institute of Surgery, Daping Hospital, The Third Military Medical University, Chongqing 400042,China） effects of RhoA/Rac balance on vascular reactivity following hemorrhagic shock(HS) in rats. Methods Hemorrhagic shock (40 mmHg for 0h or 2h) of SD rats in vivo were adopted, to observe the effects of RhoA/ Rac agonist or antagonist on mean arterial pressure (MAP), caliber of superior mesenteric artery (SMA) to NE after hemorrhagic shock. Results NE-induced the Corresponding authou: LIU Liang-ming, Email: liuliangming2002@ 基金项目：国家自然科学基金30625037）；国家973计划(2005CB522601)；教育部创新团队基金（IRT0712）；重庆市自然科学基金（CSTC2007BB5078） 通讯作者:刘良明E-mail:liuliangming2002@ pressor response and contraction of SMA following hemorrhagic shock were significantly increased at early shock (P0.01) and it was significantly decreased at late shock(P0.01). RhoA inhibitor C3enzyme and Rac activity agonist PDGF could decrease the pressor response of NE and NE induced contraction of SMA, RhoA activity agonist U-46619 and Rac inhibitor NSC23766 could increase the pressor response of NE and NE induced contraction of SMA, C3enzyme and NSC23766 abolished U-46619 and PDGF induced the change of pressor response of NE