亚麻籽木脂素对雄性大鼠肌肉生长的促进作用及机制探讨-中国农业科学.PDF

中国农业科学 2009,42(1):261-266 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.01.033 亚麻籽木脂素对雄性大鼠肌肉生长的促进作用及机制探讨 周 炜，王国杰，韩正康 （南京农业大学农业部动物生理生化重点开放实验室，南京 210095 ） 摘要：【目的】以大豆黄酮为对照，研究亚麻籽木脂素对雄性大鼠骨骼肌生长的影响并探讨其作用机制。【方 法】在SD雄性大鼠基础日粮中分别添加大豆黄酮（5 mg·kg-1 -1 ）和亚麻籽木脂素（50 mg·kg ），利用高压液相色谱、 放射免疫分析、RT-PCR等方法，分析大鼠血清中亚麻籽木脂素及两种主要代谢产物（肠内酯、肠二醇）浓度、睾 酮及生长激素含量、比目鱼肌和下丘脑中 ERβmRNA 的相对表达量。【结果】亚麻籽木脂素与大豆黄酮均能显著提 高雄性大鼠饲料利用率，促进大腿肌肉增重，提高腿肌中 RNA/DNA 的比值，促进下丘脑和比目鱼肌中 ERβ mRNA 表达量显著上升，使大鼠血清中睾酮含量增加，极显著性降低血清中尿素氮含量。【结论】亚麻籽木脂素可能通过 与ERβ结合，作用于下丘脑-垂体-性腺轴，调节大鼠血清中睾酮水平，抑制蛋白质分解，促进骨骼肌卫星细胞与 肌细胞的融合，从而促进骨骼肌生长。 关键词：植物雌激素；亚麻籽木脂素；大豆黄酮；动物木脂素；骨骼肌生长 Effects of Flaxseed Lignans on the Growth of Skeletal Muscle of Male Rats and Its Possible Mechanism ZHOU Wei, WANG Guo-jie, HAN Zheng-kang (Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture of China, Nanjing Agricultural University, Nanjing 210095) Abstract: 【Objective 】 To determine whether flaxseed lignans could affect the growth of skeletal muscle in male animals and its possible mechanisms. 【Method 】 The effect of the flaxseed lignans on skeletal muscle in male rats were determined in vivo. Daidzein (5 mg·kg-1) and flaxseed lignans (50 mg·kg-1) were added into the basal diets, respectively, and the concentrations of the serum lignans and daidzein were measured by HPLC, serum growth hormone and testosterone (T) levels were analyzed by RIA, and the expression of estrogen receptor β (ERβ) in the soleus muscle and hypothalamus were determined by RT-PCR. 【Result 】 Flaxseed lignans and daidzein significantly improved the feed effi