固定化细胞发酵半纤维素水解液产木糖醇的研究-林产化学与工业.PDFVIP

24 1 Vol.24 No. 1 2004 3 Chemistry and Industry of Forest Products Mar. 2004 黄 炜, 方祥年, 夏黎明 ( 浙江大学 化学工程与生物工程系, 浙江杭州310027) HUANG W : ( Candida tropicalis) , , , : 32 , 2.2 g3.7 g, pH 6.0, , 0~ 24 h 210 r/ min; 24~ 72 h 140 r/ min, 14 10 30 d , 73.7% , 80% , , : ; ;; : Q353. 421; Q923 :A : 0253- 2417( 2004) 01- 0029- 05 PRODUC ION OF XYLI OL FROM HEMICELLULOSE HYDROLYSA E BY IMMOBILIZED CANDIDA TROPICALIS CELLS HUANG Wei, FANG Xiang-nian, XIA L-i ming (Department of Chemical and Biochemical Engineering, hej iang University,Hangzhou 310027, China) Abstract: Candida tropicalis cells were immobilized in Ca-alginate beads and used for xylitol production from corn cob hy- drolysates. Optimum batch fermentation conditions were asfollows: 32 ; per liter nitrogen contains 2. 2 g bacto-yeast extract and 3. 7 g bacto-tryptone; initial pH value 6. 0; volume ratio between gel beads of immobilized cells and hydrolysates 14; shaking speed was divided into two stages: 210 r/ min at 0- 24 h, 140 r/ min at 24- 72 h. In 10 repeated batches(30 days) fermentation, average xylitol yieldwas 73.7% . he immobilized cells have high density, steady fermentability and good resis- tance to inhibitors in the hydrolysates. Corn cob hydrolysates can be directly converted to xylitol without being decolored or ion-exchanged. his process can effectively reduce production costs, showing a broad prospects of applications. Key words:xylitol; immobilized cells; hemicellulose hydrolysate; corn cob , , , , [ 1] , , [ 1] , ,