低频超声治疗口腔溃疡的试验研究-第三军医大学学报.docVIP

低频超声治疗口腔溃疡的动物实验研究 向学熔1，季平1，范小平1，杨明聪1，张华昌1 （401147，重庆，重庆医科大学附属口腔医院1） 摘要：目的 通过构建口腔溃疡动物模型，探讨不同照射剂量低频超声对大鼠实验性口腔黏膜溃疡愈合时间及溃疡组织中一氧化氮（NO）和一氧化氮合酶（NOS）含量的影响。方法 健康Sprague-Dawley（SD）大鼠36只，雌雄各半，随机分为6组（n=6）：1组设为正常对照组，其余5组利用氧自由基诱导法+物理烧伤法建立SD大鼠颊囊黏膜口腔溃疡动物模型，分别为溃疡组，药物组和低频超声输出功率为1.1w组、1.58w组及2.06w组。末次治疗24h后观察溃疡愈合时间，并采用分光光度计测定大鼠口腔颊囊黏膜组织中NO、NOS含量。结果 溃疡病理特点与人类口腔溃疡病理特点基本相似。低频超声组较溃疡组及药物组的溃疡愈合时间短，但低频超声各组间无显著差别。低频超声输出功率1.1W、1.58W、2.06W组较溃疡组NO、NOS活性显著降低（P0.05），与正常对照组之间统计学无意义（P﹥0.05）。结论 适合剂量参数的脉冲低频超声能有效降低组织中NO、NOS活性含量，对溃疡的愈合有一定的促进作用。 关键词： 脉冲低频超声；口腔溃疡；NO、NOS 中图法分类号： 文献标识码： Experimental study about treatment of oral ulcers with Low-frequency ultrasound Xiang Xuerong,Ji Ping,Fan Xiaoping,Yang Mingcong,Zhang Huachang (The Affiliated Stomatological Hospital,Chongqing Medical University,Chongqing,401147,China) Abstract: Objective By building an animal model of oral ulcers,to observe the effect of different energy levels of low-frequency ultrasound irradiation on the changes of NO and NOS levels in ulcer tissue of oral ulcermodel. Methods　Thirty Sprague-Daw-ley rats were inflicted with oral ulcer, and then randomly and equally assigned to 5 group s, that is, oral ulcer group, 1% iodine glycerin group and the rest 3 groups receiving low-frequency ultrasound irradiation at 1. 1W, 1. 58W, 2.06W ( once per day, for 3 consecutive days). The other 6 rats served as normal control. In 24h after last treatment, 3 rats from each group were sacrificed and their cheek poucheswere taken out for NO and NOS levels in the mucosal tissue by spectrophotomentry. Ulcer healing were observed 24h after the last treatment for 8 consecutive days. The efficiency of low-frequency ultrasound irradiation at different powers were evaluated. Results　Irradiation groups had significantly shorter healing time compared with iodine glycerin group. Level of NO and NOS activity in 1. 1W ,1. 58W ,2.06W groups was evidently lower than the ulcer group ( P 0.05) ; but the changes of NO and NOS activity had no