柿子单宁对牛血清白蛋白的荧光猝灭作用研究-河南农业科学.PDF

104 2008, Vol. 29, No. 06 食品科学 ※基础研究 1 2 1 , 1 , 3 1 1 顾海峰 ，梁晋鄂 ，李春美 * ，徐玉娟 ，陈美红 ，胡婉峰 (1.华中农业大学食品科技学院，湖北 武汉 430070 ；2. 中国食品工业协会，北京 100073 ； 3.广东省农业科学院蚕业与农产品加工研究所，广东省果蔬深加工重点实验室，广东广州 510610) ：采用荧光光谱和紫外吸收光谱法研究了柿子单宁对牛血清白蛋白(BSA) 的荧光猝灭作用。结果表明，柿 子单宁可以有规律地使BSA 内源荧光猝灭，其猝灭机理可认为是柿子单宁与BSA 形成复合物的静态猝灭，并获得 了不同温度下柿子单宁与BSA 作用的结合常数和热力学参数。根据所得结果可推断柿子单宁与BSA 的作用力为疏水 作用力和静电作用力，同时由Forster 非辐射能量转移理论计算得出了柿子单宁与BSA 结合位置的距离。 ：柿子单宁；牛血清白蛋白；相互作用；荧光猝灭 Fluorescence Quench to Bovine Serum Albumin by Persimmon Tannin 1 2 1, 1,3 1 1 GU Hai-feng ，LIANG Jin-e ，LI Chun-mei *，XU Yu-juan ，CHEN Mei-hong ，HU Wan-feng (1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China； 2. Food Industry Associations of China, Beijing 100073, China ； 3. Sericulture and Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Guangdong for Deep Processing of Fruits and Vegetables, Guangzhou 510610, China) Abstract ：The interaction between persimmon tannin and bovine serum albumin (BSA) and the mechanism of fluorescence quench were studied by fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy. The results showed that the fluorescence of BSA is quenched regularly by persimmon tannin. The binding constants and thermodynamic parameters of persimmon tannin with BSA are obtained at different temperatures. The hydrophobic interaction force and electrostatic force play a main role in the binding of persimmon tannin with BSA. The distances of binding site between persimmon tannin and BSA are also obtained according to the