COPD患者治疗前后血清NGF、TNFα和IL-18水平检测的临床意义.docVIP

精品论文 参考文献 COPD患者治疗前后血清NGF、TNFα和IL-18水平检测的临床意义 李方华1 侍步卿2 　　(1江苏省赣榆县黑林中心卫生院 222200) 　　(2江苏省赣榆县人民医院 222200) 　　【摘要】目的：探讨了COPD患者治疗前后血清NGF、TNFalpha;和IL-18水平的变化及临床意义。方法：应用放射免疫法和酶联法对35例COPD患者进行了治疗前后血清NGF、TNFalpha;和IL-18水平检测，并与30名正常健康人作比较。结果：COPD患者在治疗前血清NGF、TNFalpha;和IL-18水平均非常显著地高于正常人组（p＜0.01），经治疗一个月后则与正常人比较无显著性差异（p＞0.05），且血清NGF水平与TNFalpha;、IL-18水平呈显著正相关（r=0.5928,0.6122，p＜0.01）。结论：检测血清NGF、TNFalpha;和IL-18水平的变化对探讨其发病机制、提供了有价值的实验室依据。 　　【关键词】慢性阻塞性肺疾病 血清生长因子 肿瘤坏死因子alpha; 白细胞介素—18 　　【中图分类号】R563.9 【文献标识码】A 【文章编号】2095-1752（2014）11-0034-02 　　Clinical Significance of changes of Determination of Serum NGF、TNFalpha;and IL-18 Levels after treatment in patients With chronic obstructive pulmonary diseases(COPD). 　　1. Jiangsu ganyu County heilin cetre santiory 2 .Jiangsu County in Ganyu province peoples Hospital 　　Li Fanghua1 Shi Buqing2 　　【Abstract】Objective To explore The clinical Significance of Changes of Serum NGF、TNFalpha; and IL-18 levels after treatment in patients With COPD.Methods Serum TNFalpha;(With RIA),Serum NGF、IL-18 (With ELISA) levels were determined in 35 patients With COPD and 30 controls.Results Before treatment, Serum NGF、TNFalpha; and IL-18 levels were Significantly higher than those in Controls（p＜0.01）.After treatment for 1 month,the levels showed no Significant different from the Controls (p＞0.05).Serum NGF level expersed positively correlated With Serum TNFalpha;、IL-18 levels（r=0.5928、0.6122，p＜0.01）.Conclusions Detection of Serum NGF、TNFalpha;and IL-18 levels after treatment provides a valuable taboratory basis for the explortion of the pathogenesis in COPD. 　　【Key words】nchronic obstructive pulmonary diseases(COPD) Neveros growth factor(NGF) tumor necrosis factor-alpha;(TNF-alpha;) interleukin-18(IL-18) 　　慢性阻塞性肺病（COPD）为气道慢性炎症性疾病，其发病机制十分复杂，可能与炎症、遗传、感染、环境因素和TH1/TH2细胞亚群失衡等密切相关[1][2]。本文对35例COPD患者进行了治疗前后血清NGF、TNFalpha;和IL-18的测定，并就临床价值作初步探讨，现将我们的结果报告如下： 　　1 资料与方法