兖州卷柏总黄酮提取方法的研讨.pdfVIP

兖州卷柏总黄酮提取方法的研究 张昊 ，黄可龙 ，施树云，鲁曼霞，刘素琴 （中南大学化学化工学院，湖南长沙 410083） 摘要: 作为一种常用的民间草药，兖州卷柏等卷柏属植物都具有止血、抑菌、消炎、抗肿瘤、抗突变、降 血糖、免疫和镇痛等药理活性。为了研究其活性成分的最佳提取条件，采用多种方法对比后，优选微波辅 助提取法，在单因素实验的基础上，通过正交试验，确定了兖州卷柏总黄酮的最优提取条件。结果表明： 以70％乙醇为溶剂，料液比为1：20，功率为462W时，用微波辅助法提取10min为最优提取条件。 关键词：兖州卷柏；总黄酮；提取；正交实验 Optimization of the Extraction Techniques of Total Flavonoids from Selaginella involvens （Sw.）Spring Zhang Hao ，Huang Ke Long ，Shi Shu Yun，Lu Man Xia ，Liu Su Qin (College of Chemistry Chemical Engineering,，Central South University ，Changsha ，410083) Abstract ：The total flavonoids were extracted with alcohol as the solvent from Selaginella involvens （Sw. ）Spring through traditional diffusion extraction, soxhlet extraction, ultrasonic extraction and microwave-assisted extraction. Investigated the various factors effecting on extraction of total flavonoids，such as the concentration of alcohol, the ratio of medicinal herb-alcohol, the time of extraction and the microwave power .Based on the experiments of single factor, the optimum extraction conditions were definited by orthogonal experiment.The result showed that the optimal yield rate was 3.41%,as long as the concentration of alcohol was 70%, the microwave power was 462w,the ratio of medicinal herb-alcohol was 1:20,and the time of extraction was 10 min on the method of microwave-assisted extraction. Key words ：Selaginella involvens （Sw. ）Spring；total flavonoids ；extraction 1 引言 兖州卷柏(Selaginella involvens （Sw. ）Spring)，多年生草本，异名金不换、茯苓蕨、扇 卷柏等[1] 。作为一种常用的民间草药，兖州卷柏与被《中国药典》收载的卷柏S. tamariscina (Beauv.) Spring、垫状卷柏S.pulvinata (Hook.et Grev.) Maxim 等卷柏属植物都具有止血、抑菌、 消炎、抗肿瘤、抗突变、降血糖、免疫和镇痛等药理活性[2-4] ，。尤其是双黄酮的药理作用是 多方面的，大量文献对此进行了报道[5-14] ，为了进一步开发其工业级药用价值，本文分别用 传统的浸提、索氏提取、微波辅助提取和超声提取法用对兖州卷柏的总黄酮的提取及其工艺 进行了系统研究，对兖州