幼苗活性氧代谢对南方根结线虫侵染的反应-热带亚热带植物学报.pdfVIP

热带亚热带植物学报 2016,24(1)：63~70 Journal of Tropical and Subtropical Botany 番茄‘Ls-89’和‘坂砧2号’幼苗活性氧代谢对南方根 结线虫侵染的反应 1,2 2 * 贾双双 , 徐坤 (1. 安徽科技学院生命科学学院，安徽 凤阳 233100 ；2. 山东农业大学园艺科学与工程学院，山东 泰安 271018) 摘要：为了解番茄(Lycopersicon esculentum)砧木幼苗活性氧代谢与抗南方根结线虫(Meloidogyne incognita) 的关系，以高感品 种‗Ls-89‘与高抗品种‗坂砧2 号‘为材料，采用盆栽人工接种法，研究了南方根结线虫侵染对番茄砧木幼苗活性氧代谢的影响。 结果表明，未接种南方根结线虫的番茄砧木幼苗，其根系与叶片活性氧水平及相关酶活性在品种间没有显著差异。接种南方 – 根结线虫后，两品种幼苗根系与叶片的O ·生成速率和H O 含量均升高，且‗坂砧2 号‘显著高于‗Ls-89‘，但‗Ls-89‘的MDA 2 2 2 含量则显著高于‗坂砧2 号‘。两品种幼苗根系与叶片的SOD 活性均在侵染早期降低，以‗坂砧2 号‘降幅较大；但POD 、CAT 活性在侵染早期变化不大，至侵染中后期则显著升高。因此，番茄抗性品种砧木幼苗的膜脂抗氧化能力较强，活性氧水平较 高，且SOD 活性对南方根结线虫侵染敏感。 关键词：番茄；砧木；南方根结线虫；活性氧；保护酶活性 doi: 10.11926/j.issn.1005-3395.2016.01.009 Reactive Oxygen Metabolism and Response to Meloidogyne incognita Infection in Tomato ‘Ls-89’ and ‘Banzhen 2’ Rootstock Seedlings JIA Shuang-shuang1,2, XU Kun2* (1. College of Life Science, Anhui Science and Technology University, Fengyang 233100, Anhui, China; 2. College of Horticulture Science and Engineering, Shandong Agricultural University, Tai‘an 271018, Shandong, China) Abstract: In order to understand the relation between reactive oxygen (ROS) metabolism of tomato (Lycopersicon esculentum) rootstock seedlings and their resistance to Meloidogyne incognita , a high sensitive rootstock ‗Ls-89‘ and a high resistant rootstock ‗Banzhen 2‘ were planted in pots and inoculated with M. incognita, the effect of M. incognita infection on ROS metabolism and related enzyme activities in tomato rootstock seedlings was studied. The results showed that ROS level and related enzyme activities in roots and leaves had no significant difference between two varieties uninfected by M. inc