噬菌体及其裂解酶对细菌生物被膜作用的研究进展.pdfVIP

微生物学通报 Mar. 20, 2015, 42(3): 568−573 Microbiology China © 2015 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.140403 专论与综述 噬菌体及其裂解酶对细菌生物被膜作用的研究进展 1 1 1 1 1,2* 吕芸辉 全心馨 沈梦溪 易秋雪 崔泽林 (1. 200025) (2. 200080) 摘 要细菌形成的生物被膜，可保护细菌不易被抗生素杀死，这给临床上相应疾病的治疗及 医疗器械的消毒带来极大困难。研究表明，噬菌体及其裂解酶对生物被膜有降解作用。噬菌体 能清除细菌在有生物活性或无生物活性的介质表面形成的生物被膜。此外，噬菌体裂解酶比如 LySMP 、肽酶CHAPk 、细胞壁溶解酶CWHs 等能清除特定的生物被膜，这可能与裂解酶直接 溶菌和裂解细菌细胞外基质有关。同时，与抗生素、钴离子、氯等物质联合使用时，噬菌体对 生物被膜的清除作用会更强。本文从噬菌体、噬菌体编码的裂解酶、以及它们联合其他物质对 细菌生物被膜的作用进行综述，并对其实际应用做了展望。 关键词噬菌体，裂解酶，细菌生物被膜 Research progress in biofilm degradation by bacteriophage and its lysin LÜ Yun-Hui1 QUAN Xin-Xin 1 SHEN Meng-Xi 1 YI Qiu-Xue 1 CUI Ze-Lin1,2* (1. Department of Immunology and Microbiology , School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China) (2. Department of Laboratory Medicine of the First Affiliated People’s Hospital of Shanghai Jiao Tong University, Shanghai 200080, China) Abstract: The biofilms formed by bacteria can protect them from antibiotics, it also can bring difficulties for the treatment of infectious diseases and the sterilization of clinical machines. Recent research progress showed that bacteriophages and their lysins are potential use for eradication of biofilm, which is of great significance in control of various infectious diseases clinically. Bacterioph