应用生物信息学筛选糖尿病肾病标志物.docVIP

精品论文 参考文献 应用生物信息学筛选糖尿病肾病标志物 杨丹1 曾建涛2 郑兆斌3 　　（1.重庆市长寿区中医院肾病科 重庆 长寿 401220） 　　（2.重庆市长寿区人民医院检验科 重庆 长寿 401220） 　　（3.重庆市长寿区人民医院心内科 重庆 长寿 401220） 　　【摘 要】目的：从分子网络水平筛选糖尿病肾病诊断的标志物。方法：在GEO数据库下载糖尿病肾病的基因表达数据，应用Array-Tools软件分析差异表达基因，STRING工具用于构建差异蛋白的相互作用网络。结合组织特异表达数据库TiGER，筛选肾脏组织相关的HUB蛋白作为糖尿病肾病生物标志物。结果：肾小球组织有906个差异表达基因，其中上调基因689个，下调基因217个；肾小管组织有828个差异表达基因，其中上调基因266个，下调基因562个。通过网络构建及组织特异性筛选，在肾脏组织差异表达的HUB蛋白为GZMA、SPP1,TGFBI,FGL2,KNG1,VCAM1和EGF。结论：通过生物信息学研究，筛选出7种蛋白质可能成为糖尿病肾病诊断的标志物。 　　【关键词】糖尿病肾病；生物信息学；生物标志物 　　【中图分类号】R587.2 【文献标识码】A 【文章编号】1003-5028（2015）8-0679-02 　　Screening the biomarkers of diabetic 　　nephropathy by bioinformatics methods 　　Yang Dan1，Zeng Jian tao2，Zheng Zhao bin3 　　（1.Department of Nephrology,The traditional Chinese medical hospital of Changshou, Chongqing, 401220） 　　（2.Department of Clinical Laboratory,The Peoplersquo;s Hospital of Changshou, Chongqing, 401220） 　　（3.Department of cardiology,The Peoplersquo;s Hospital of Changshou, Chongqing, 401220） 　　【Abstract】Objective: To screen biomarkers of diabetic nephropathy in Biomolecular Network level.Methods: Gene expression data of kidney were got from Gene Expression Omnibus database. Array-Tools software was used to analyze differential expression gene. Then The protein-protein interaction (PPI) networks of differential expression gene were constructed by STRING. Considering the database of TiGER, the HUB proteins which express specificity in kidney were filted as biomarkers of diabetic nephropathy.Results: 906 differentially expressed genes were indentified in glomcrulus, including 689 up-regulated and 217 down-regulated genes. 828 differentially expressed genes were indentified in renal tubule, including 266 up-regulated and 562 down-regulated genes. Several HUB proteins (GZMA、SPP1,TGFBI,FGL2,KNG1,VCAM1和EGF) were discovered by PPI networks constructing and tissue-specific filting.Conclusion: Our study acquired 7 proteins by bioinformatics, these proteins maybe underlying diagnosis bi