胰岛素调节骨骼肌细胞葡萄糖转运子4内在活性的研讨.pdf

胰岛素调节骨骼肌细胞葡萄糖转运子4内在活性的研究 牛文彦姚智 【摘要】目的探讨胰岛素刺激骨骼肌摄取葡萄糖的机制。方法观察GLUT4转位和葡萄糖 摄取对SB203580和Wortmannin的响应，以及胰岛素在分化前后的细胞中对两者的作用， 研究胰岛素信号通路。结果胰岛素分别增加GLUT4转位和葡萄糖摄取2．55：0．2倍2．25：0．1 GLUT4的标记，但不影响GLUT4转位；胰岛素刺激前骨骼肌细胞葡萄糖摄取增加的倍数 骼肌细胞中存在两个胰岛素信号转导途径，分别介导GLUT4的转位和活化，胰岛素利用这 两条信号通路达到最大的刺激细胞摄取葡萄糖的作用。 【关键词】 葡萄糖转运子4；葡萄糖摄取：转位；内在活性 The ofIntrinsic ofGlucose 4 InsulininSkeletal Study Activity TransporterRegulatedby MuscleCells Niu，Zhi Medical Medical IWenyan Yao(Dept，ofImmunology,Tianjin University,Tianjin300070) To themechanismofinsulin—stimulated inskeletal [Abstract]Objectivestudy glucoseuptake musclecells．MethodsTo insulin the ofGLUT4 signal study pathwaybydetectingresponses translocationand to 580and inaswellas of on SB203 wortmann theeffectinsulin glucoseuptake thesetwoevens differentiation．ResultsBothofGLUT4translocationand during glucose werestimulated and2．25：0．1foldVS is transport byinsulin，2．5±o．2 control，respectively；buttl，2 3．3min to and6．0rain．and wortmanninis43nMand3nM inhibits ICso respectively．SB203580 64％and62％ofinsulin—stimulated and ofcellsurface glucoseuptakephotolabelling GLUT4， noeffectonGLUT4translocation．Thefoldincreaseofinsulin- respectively,but stimulated foldVScontr01)islowerthanthatofGLUT4translocation uptake(1．7±0．1 glucose foldVS Inskeletalmuscle insulin (2．35：0．1 contr01)inmyoblasts．Conclusioncells，two signal mediateGLUT4translocat