娄彻氏链霉菌产酵母胞壁溶解酶发酵条件的研讨.pdfVIP

J026 娄彻氏链霉菌产酵母胞壁溶解酶发酵条件的研究∗ 1 1 1 1 2 2 2 蹇华丽 朱明军 吴振强 梁世中 姚朔影 梁淑娃 夏枫耿 (1 华南理工大学食品与生物工程学院，广州 510640 2 广州市微生物研究所，广州 510250) 摘 要 娄彻氏链霉菌（Streptomyces rochei A4.73）产胞壁溶解酶的适宜的碳源为酵母细胞 壁，氮源为（NH ）SO ，接种量 10%， 250ml 三角瓶装液量为 30ml，发酵温度为 25℃，培养基初 4 2 4 始 pH 7.5。在此条件下发酵 96h 后可达到最高酶活 95.4u/ml，比条件优化前的酶活提高了 86.3%。 将所得的发酵液用于红法夫酵母虾青素的酶法提取，总类胡萝卜素的提取率达到 98%以上。 关键词 胞壁溶解酶，娄彻氏链霉菌，发酵条件 STUDY ON THE FERMENTATION CONDITIONS OF STREPTOMYCES ROCHEI A4.73 PRODUCING LYTIC ENZYME Jian Huali1 Zhu Mingjun1 Wu zhenqiang1 Liang Shizhong1 Yao shuoying2 Liang Shuwa2 Xia Fenggeng2 (1 The College of FoodBiotech. Eng., South China Univ. Of Tech., Guangzhou,510640 2 Guangzhou Microbe Research Istitute, Guangzhou,510250) Abstract: The effects of different factors on lytic enzyme production by Streptomyces rochei A4.73 were studied in shake flask condition. Yeast cell wall was the best C sources and （NH ） SO was the 4 2 4 best N sources. The best inoculum concentration was 10% and 30ml medium volume in 250ml triangle flask was benefit for Streptomyces rochei A4.73 to produce lytic enzyme which had high enzyme activity. The fermentation temperature and the best initial pH value of growth medium were 25 ℃ and 7.5, respectively. The lytic enzyme activity of fermentation broth reached 95.4u/ml when Streptomyces rochei A4.73 was cultured on above-mentioned conditions, increasing 86.3% contrasted to the initial. This fermentation fluid was used for extracting astaxanthin of Phaffia rhodozyma . When reaction temperature and pH for carotenoid extraction was 30 ℃ and pH 6.5, respectively, within 30h, over 98% of the to