反相高效液相色谱法同时测定止痒酊中间苯二酚、水杨酸、苯酚的含量研究.pdfVIP

反相高效液相色谱法同时测定止痒酊中间苯二酚 水杨酸 苯酚的含量 邢蓉 邓子煜 陈根德 蚌埠医学院附属医院 安徽蚌埠 233004 [摘要] 目的 测定止痒酊中间苯二酚 水杨酸 苯酚的含量 方法 反相高效液相色谱法 色谱柱 为Lichrospher C18 柱(4.6mm250mm,5m) 流动相为甲醇-水 48 52 流速1.0ml·min- 1, 检测波长 -1 为 270nm 结果 一次进样即可完成三种成分的含量分析 间苯二酚 40 200mg· L 水杨酸 20 -1 -1 100mg·L 苯酚20 100mg· L 范围内 峰面积与浓度呈良好的线性关系 日内 日间RSD 在0.62% 2.58% 高 中 低浓度回收率在 96.83% 103.98% 结论 本方法简便 快速 准确 可作为止痒 酊的质量控制方法 [关键词] 止痒酊 间苯二酚 水杨酸 苯酚 反相高效液相色谱法 RPHPLC determination of resorcinol and salicylic acid and phenol in antiprurit tincture Xing Rong, Deng Zi-Yu, Chen Gen-De (The Affiliated Hospital of Bengbu Medical Colloge, Anhui Bengbu 233004) ABSTRACT OBJECTIVE To determine three componentr of resorcinol and salicylic acid and phenol in the tincture. METHODS RPHPLC method was used. ,the chromatographic conditions were as follows: Lichrospher C18 column(4.6mm250mm,5m), methanol and water(48:52) as mobile phase, at a flow rate of 1.0 ml·min- 1, detection wavelength 270 nm. RESULTS The determination of three components was - 1 accomplished by only one injection of sample. The calibration curves were linear in range of 40 200mg·L -1 -1 of resorcinol and 20 100mg· L of salicylic acid and 20 100mg· L of phenol. The RSD of intra-day and inter-day was within 0.62% 2.58%.The recovery of high,medium and low concentration was within 96.83% 103.98%. CONCLUSIONS The method was simple,rapid and accurate. It can be used for the quality control of antiprurit tincture. KEY WORDS antiprurit tincture; resorcinol; salicyl