胆固醇溶液的光谱学研讨.pdfVIP

1,a a, b a, b b a a 210094 b 210099 409 nm LED 473 nm LED Fluorometic Determination of Cholesterol Solution a a, b a, b b a LAN Xiufeng LIU Jian’gang LUO Xiaosen TANG Xin’guo NI Xiaowu a Department of Information Physics and Engineering, Nanjing University of Science Technology, Jiangsu Nanjing, 210094 b Nanjing Medical College, Second Military Medical University of PLA, Jiangsu Nanjing, 210099 Abstract: Spectroscopy of cholesterol solution is studied by the technology of fluorescence analysis in this paper. The experimental results indicate that cholesterol solution can emit strong fluorescence under the excitation of LED light with the wavelength at 409 nm. The peak of profile locates about 473 nm and the fluorescence intensity is linear with the concentration of cholesterol solution. By comparing the fluorescence intensity, the content of cholesterol can be educed. The research results suggest that it is viable to apply the technology of fluorescence analysis to test the content of cholesterol solution. And the consequences in this paper may offer an experimental reference to study the fluorescence characteristic of cholesterol in blood and to develop a new method of blood detection. Key words: cholesterol, fluorescence spectra, LED light induced, hypercholesterolemia