检测结直肠癌组织中miR-21，miR-106a，miR-143的表达水平.docVIP

精品论文 参考文献 检测结直肠癌组织中miR-21，miR-106a，miR-143的表达水平 彭志平 刘紫玲 史学森（通讯作者） 　　【摘 要】 目的 探讨良性结肠腺瘤与结肠腺癌组织中Hsa-miR-21-5p，Hsa-miR-143-3p，Hsa-miR-106a-5p表达水平的差异对临床诊断结肠腺瘤及癌变可能的意义。方法 利用荧光实时定量PCR（qRT-PCR）检测结肠腺瘤、结肠癌患者组织及瘤旁组织中Hsa-miR-143-3p，Hsa-miR-21-5p，Hsa-miR-106a-5p的表达水平，并分析三者的表达对结肠腺瘤或结肠癌的诊断意义。结果 结肠腺瘤组织中Hsa-miR-21-5p，Hsa-miR-106a-5p的表达水平较瘤旁组织显著增高（plt;0.05），Hsa-miR-143-3p的表达水平显著降低（plt;0.05）；经病理鉴定癌变的组织中Hsa-miR-21-5p表达水平显著高于良性瘤组织，差异有统计学意义（plt;0.05），而Hsa-miR-106a-5p的表达水平与良性瘤组织无统计学差异（pgt;0.05)，Hsa-miR-143-3p的表达水平低于良性瘤组织，差异有统计学意义。结论 Hsa-miR-21-5p，Hsa-miR-106a-5p，Hsa-miR-143-3p的表达水平对临床诊断结肠腺瘤及是否发生癌变有指导意义。 　　【关键词】 结肠腺瘤； 结肠腺癌； miRNA； Hsa-miR-21-5p； Hsa-miR-106a-5p； Hsa-miR-143-3p 　　The expression and significance Hsa-miR-21-5p, Hsa-miR-106a-5p, Hsa-miR-143-3p in colorectal adenomas 　　【Abstract】 Objective Detecting the expression of Hsa-miR-21-5p, Hsa-miR-106a-5p, Hsa-miR-143-3p in colorectal adenomas and colon adenocarcinoma, to investigate their clinical diagnosis significance in colon adenoma and colon adenocarcinoma. Methods Fluorescence quantitative real-time PCR (qRT-PCR) assay were used to detect the expressions of Hsa-miR-143-3p, Hsa-miR-21-5p, Hsa-miR-106a-5p in colorectal adenoma tissues and Adenomatous adjacent tissues, further reveal their roles during colon adenoma or colon cancer diagnosis on the basis of corresponding pathological diagnosis. Results Hsa-miR-21-5p , Hsa-miR-106a-5p expression levels in colorectal adenomas than tumor adjacent tissues was significantly higher (plt;0.05), the expression level of Hsa-miR-143-3p significantly lower (plt;0.05); the expression Hsa-miR-21-5p was significantly higher than benign tumor tissue, the difference was statistically significant (plt;0.05), but Hsa-miR-106a-5p was not (pgt;0.05), Hsa-miR-143-3p expression level lower than benign tumor tissue, the difference was statistically significant. Conclusion It is meaningful to detect the expression of Hsa-miR-21-5p,Hsa-miR-106a-5p, Hsa-miR-143-3p for clinical diagnosis o