沉渣包埋切片及免疫组化检测在胸水诊断中的应用价值.docVIP

精品论文 参考文献 沉渣包埋切片及免疫组化检测在胸水诊断中的应用价值 林建龙 钟国栋 王婷 林晶 魏霖 程焰红 　　（福建中医药大学附属第二人民医院病理科 福建 福州 350003） 　　【摘要】 目的：探讨液基细胞学联合沉渣包埋切片及免疫组化检测在胸水诊断中的应用价值。方法：对368例胸水进行液基细胞学检测，其中65例恶性及可疑恶性胸水进行沉渣包埋切片，并联合应用免疫组化染色，进一步明确诊断及追溯其组织来源。结果： 65例胸水液基细胞学检测阳性49例（75.38%），可疑阳性16例，经沉渣包埋切片及免疫组化检测出阳性61例，阳性率93.85%，差异有统计学意义(P＜0.05)；经免疫组化标记结果证实，肺腺癌41例、肺鳞癌2例、肺神经内分泌癌1例、恶性间皮瘤2例、胃肠道癌9例、乳腺癌2例、胆管癌1例、卵巢癌3例、反应性间皮增生4例。结论：液基细胞学联合沉渣包埋切片及免疫组化检测，可提高胸水细胞学诊断准确性和阳性检出率, 并对判断肿瘤细胞组织来源具有临床应用价值。 　　【关键词】 液基细胞学；沉渣包埋切片；免疫组织化学；胸水 　　【中图分类号】R446 【文献标识码】A 【文章编号】2095-1752（2015）26-0107-03 　　Application Value of Sediment Embedding Biopsy And Immunohistochemical Detection In Hydrothorax 　　Lin Jianlong, Zhong Guodong, Wang Ting, Lin Jing,Wei Lin,Chen Yanhong 　　 Department of pathology ,The second peoples hospital affiliated to fujian university of traditional Chinese medicine. Fuzhou China 350003 　　【Abstract】Objective To explore the application value of Liquid based cytology combine with sediment embedding biopsy and immunohistochemical detection in the diagnosis of hydrothorax.Methods To detecte liquid based cytology of 368 cases of hydrothorax and we found that 65 cases were malignant or suspicious malignant，and we further diagnosis and trace their organization sources by the means of sediment embedding biopsy combined with immunohistochemical staining.Results By the method of liquid based cytology ,we found that 49 cases (75.38%) were positive and 16 cases were suspicious positive . On the other hand ,by the method of sediment embedding biopsy conbined with immunohistochemical detection , 61 cases were positive,with the positive rate of 93.85%,and the difference was statistically significant (p lt; 0.05). Immunohistochemical markers results Confirmed that 41 cases of patients were lung adenocarcinoma, 2 cases were lung squamous carcinoma,1case was pulmonary neuroendocrine carcinoma, 2 cases were malignant mesothelioma, 9 cases were gastrointestinal carcinoma, 2 cases were breast cancer