大鼠宫内感染模型头孢曲松钠宫内静脉给药对胎盘-第三军医大学学报.docVIP

宫内给药对大鼠细菌性宫内感染胎盘IL-6与MMP-9表达的影响 刘 建，程 浩，邹姝丽，兰 易，甘小玲 （重庆医科大学附属第二医院妇产科，重庆400010） 提要：目的 探讨头孢曲松钠羊膜腔内和静脉给药对大肠埃希菌所致宫内感染的晚孕SD大鼠胎盘组织白细胞介素-6（IL-6）-9（MMP-9）15天的SD孕鼠36只，完全随机分成4组，每组9只。（1）妊娠第15天，所有孕鼠一侧宫角行1010/L大肠埃希菌菌液0.2ml接种。（2）妊娠第16天，羊膜腔内给药组（IUA）和对照组（IUC）分别于接种侧各羊膜腔内注射头孢曲松钠和等体积的生理盐水，静脉给药组（IVA）和对照组（IVC）母鼠尾静脉分别注射头孢曲松钠和等体积的生理盐水。（3）四组孕鼠自妊娠第17天起，每组每天随机处死3只，随机抽取其接种侧宫角顶端三个胎囊之一的胎盘行IL-6、MMP-9SD大鼠大肠埃希菌宫内感染，羊膜腔内给药较母鼠静脉途径更有效，其可通过抑制IL-6和MMP-9在胎盘组织中的表达水平，降低炎症反应水平和胎膜早破的风险。 关键词：绒毛膜羊膜炎，大肠杆菌性；妊娠末期；投药，羊膜腔内；白细胞介素6；基质金属蛋白酶9 中图法分类号： R714.5 文献标识码：A Intraamniotic administration on placental IL-6 and MMP-9 expressions in rats with intrauterine infection of E. coli LIU Jian, CHENG Hao, ZOU Shu-li, LAN Yi, GAN Xiao-ling (Department of Obestetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China) Abstract: Objective To explore the effect of ceftriaxone sodium administered intraamniotically and by maternal intravenous injection on the expressions of placental interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) in treatment of intrauterine infection with E. coli in late pregnant SD rats. Methods Thirty-six SD rats on gestation day 15 were randomly divided into four groups (9 in each). On gestation day 15, 0.2 ml E. coli suspension at a dose of 1010/L was inoculated into one horn of uterus; On gestation day 16, each gestational sac in inoculated side of rats in intrauterine administration (IUA) group and control (IUC) group was given ceftriaxone sodium or appropriate volume of isotonic Na chloride (NS) intraamniotically, and each rat in maternal intravenous injection (IVA) group and control (IVC) group was given ceftriaxone sodium or appropriate volume of isotonic NS intravenously. From the gestation day 17 on, 3 rats of each group were sacrificed randomly each day. One of the top 3 sacs in inoculated side of each rat was randomly selected, and the expressions of IL-6 and MMP-9 were analyzed semi-quantitively by