酶法辅助提取南板蓝叶中靛玉红的工艺研究.pdfVIP

Anhui 安徽农业科学，JoumaI。fAgn．Sci．2012，40(26)：12866—12867，1287．9 责任编辑石金友责任校对卢瑶 酶法辅助提取南板蓝叶中靛玉红的工艺研究 黄凤婷1，马永良2，林春颖1，熊清平3+ 院，广东广州510120；3．淮阴工学院，江苏淮安223003) 摘要[目的]研究酶法提取关键参数对南板蓝叶中靛玉红提取工艺的影响。[方法]采用HPLC测定靛玉红的含量，并考察酶用量、酶 解pH值和酶解时间对提取率的影响，并优选出了最佳工艺条件。[结果]南板蓝叶中靛玉红酶法辅助提取的最佳工艺条件为：酶用量 25U／g，酶解pH值4．8，酶解时间6 h；在此条件下，靛玉红的z值为7．02％。[结论]酶法辅助提取法适用于南板蓝叶中靛玉红的提取。 关键词酶法辅助提取；靛玉红(Indirubin)；南板蓝根-(Baphicacanthuscuria(Nees)Bremek) 中图分类号S567文献标识码A 文章编号0517～6611(2012)26—12866—02 on of ExtractionIndirubinfrom CUSia(NeeslBremekLeaves StudyEnzyme-assisted BaphicacanthHs HUANG et Branchofthe3rdAffiliated of of Chinese Traditional Feng-tingal(Fangcun hospitalGuangzhouUniversity Medicine，Guang． zhou，Guangdong510360) Abstract Toobservethe of that extraction extractionofindirubinfrom cusia [Objective keyparametersenzymatic impact Baphicaeanthu5 contentofindirubinwasdetermined of and (Nees)Bremekleaves．[Method]TheusingHPLC．Effectsenzymedosage，hydrolysispH hydrol— timeonextractionrateofindirubinwere withtheaimof itsextraction ysis investigated optimizing technology．[Result]Theoptimalenzyme—as— extraction from sisted conditionsofindirubinB．cusialeaveswerethecombinationof 25 4．8and enzymedosageU／g．hydrolysispH hydrolysis time6h．Undertheabove Zvalueofindirubinwas extractionmethodiSsuitablefor conditions，the 7．02％．1 Conclusion]Enzyme—assisted of extractionindirubinfromB．cusia1eaves． words Key Enzyme—assistedextraction；Indirubin：Baphicacanthuscusia(Nees)Bremek 靛玉红(