苦参碱对人皮肤癌A431细胞增殖与细胞周期的影响.docVIP

精品论文 参考文献 苦参碱对人皮肤癌A431细胞增殖与细胞周期的影响 黑龙江省哈尔滨市中医医院 150076 摘要：目的：观察苦参碱对人皮肤癌A431细胞体外增殖、细胞周期的影响，探讨其对皮肤癌可能的作用机制。方法：MTT法检测苦参碱对人皮肤癌细胞增殖，流式细胞仪检测苦参碱对人皮肤癌细胞周期的影响。结果：苦参碱在0.5、0.75、1g/L浓度时均能明显抑制人皮肤癌A431细胞增殖，抑制率分别为13.35%、23.61%和41.47%，与对照组相比差异显著（Plt;0.01），IC50为1.21 g/L。苦参碱0.5、0.75、1g/L G0/G1期细胞数量明显高于对照组，并呈浓度依赖关系。结论：苦参碱对人皮肤癌细胞有明显的生长抑制作用，且有剂量依赖关系；苦参碱可能是通过将人皮肤癌细胞周期阻滞在G0/G1期，抑制其增殖，发挥其抗皮肤癌作用。 关键词：苦参碱；人皮肤癌细胞；细胞增殖；细胞周期；凋亡 Effects of matrine on cell proliferation，cell cycle of human skin cancer cell line A431 Abstract：Objective To investigate the effects of matrine on cell proliferation，cell cycle and apoptosis rate of human skin cancer cell line A431.Methods MTT was used to measure inhibitory effects of matrine on growth of A431 cells，flow cytometry was used to analyze the cell cycle and apoptosis rate．Results：Matrine can obviously inhibited the growth of A431 at the concentration of 0.5，0.75 and 1 g/L，and the inhibiting rates were 13.35%、23.61% and 41.47%，respectively，which was in a dose-dependent manner，and there were significant difference in comparison with the control group（Plt;0.01）.IC50 value was 1.21 g/L.The number of A431 cells in G0/G1 phase of matrine groups were significantly more than that in the control group and in a concentration-dependent manner.Conclusion：Matrine had a significant inhibiting effect on human skin cancer cells in an obvious dose-dependent manner.Matrine inhibited the proliferation of the cells by blocking the skin cancer cell cycle to G0/G1 phase，and developed its anti-tumor function by inducing human A431 cells apoptosis. Key words：Matrine；human skin cancer A431 cells；cell proliferation；cell cycle；apoptosis 引言 苦参系豆科植物苦参的干燥根。其含有多种生物碱，但主要为苦参碱和氧化苦参碱[1]。苦参碱具有抗病毒、抗心律失常、抗炎、抗肿瘤、抗纤维化等多种药理作用[2-5]，广泛应用于临床。本文针对苦参碱对人皮肤癌细胞增殖、细胞周期及细胞凋亡作用的影响，探讨其对皮肤癌可能的作用机制。 1 材料和方法 1.1 材料 人皮肤癌细胞株A431购自中科院上海细胞库。 1.2 试剂 苦参碱（西安东华生物工程有限公司），顺铂（浙江海正药业），RPMI1640 培养基（美国GIBCO产品）；胎牛血清（杭州四季青）；MTT、PI染料、DMSO均购自Sigma公司，Annexin V-FITC细胞凋亡检测试剂盒购自北京鼎国生物技术公