蝮龙抗栓丸对脑缺血再灌注损伤大鼠脑组织TNF-α表达的影响.docVIP

精品论文 参考文献 蝮龙抗栓丸对脑缺血再灌注损伤大鼠脑组织TNF-α表达的影响 宋慧峰 　　（辽宁中医药大学/沈阳市第二中医院 辽宁 沈阳 110000） 　　【摘要】目的：观察蝮龙抗栓丸对脑缺血再灌注损伤大鼠脑组织中肿瘤坏死因子-alpha;(TNF-alpha;）含量的影响。方法：利用完全随机法将75只SD大鼠分为：假手术组（15只）、模型组（15只）、治疗I组（15只）、治疗Ⅱ组（15只）、对照治疗组（15只），予以治疗I组蝮龙抗栓丸0.65 mg/g、治疗Ⅱ组蝮龙抗栓丸1.29 mg/g、对照治疗组银杏叶软胶囊0.13 mg/g，灌胃1次/天,共计7天。末次灌胃后应用线栓法制备脑缺血再灌注大鼠模型，采用ELISA方法测定各组大鼠脑组织中TNF-alpha;含量的变化。结果：与模型组比较，各治疗组大鼠脑组织TNF-alpha;含量比模型组降低（P＜0.05）。结论：蝮龙抗栓丸具有明显改善大鼠脑缺血再灌注损伤，其机制与降低脑缺血区域中TNF-alpha;含量有关。 　　【关键词】蝮龙抗栓丸；脑缺血再灌注；肿瘤坏死因子-alpha; 　　【中图分类号】R453 【文献标识码】A 【文章编号】2095-1752（2015）03-0154-02 　　Fu dragon antithrombotic pill on cerebral ischemia reperfusion injury in rat organization influence of TNF alpha expression Song Huifeng.The second hospital in Shenyang, Liaoning province, Shenyang 110000, China 　　【Abstract】Objective Fu dragon antithrombotic pill on cerebral ischemia reperfusion injury in rats had the effect of tumor necrosis factor levels in the organization.Methods With completely random method, 75 SD rats can be divided into: the control group (15), model group (15), treatment group I (15), treatment group II (15), contrast the treatment group (15), the treatment group I Fu dragon antithrombotic pill 0.65 mg/g, treatment group II Fu dragon antithrombotic pill 1.29 mg/g, contrast treatment group ginkgo biloba soft capsule 0.13 mg/g, lavage 1 time/day, a total of 7 days. After lavage application line plug at the end of the preparation of cerebral ischemia reperfusion model in rats, using ELISA method in the determination of each group rats had the change of the content of TNF alpha.Results Compared with model group, the content of TNF alpha treatment group rats had lower than model group (p lt; 0.05).Conclusions Fu dragon antithrombotic pill has obvious improvement in ischemia reperfusion injury in rats and its mechanism with lower content of TNF alpha in the ischemic region. 　　【Key words】Fu dragon antithrombotic pill; Cerebral ischemia reperfusion; Tumor necrosis factor alpha 　　肿瘤坏死因子（TNF-alpha;）是一种